Detection and molecular characterization of Shiga toxin producing Escherichia coli (STEC) autoagglutinating adhesion gene (saa) from piglets in Mizoram

J. L. Kataria; T. Dutta; P. Roychoudhury; Jully Gogoi Tiwari

doi:10.14202/vetworld.2014.373-376

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Detection and molecular characterization of Shiga toxin producing Escherichia coli (STEC) autoagglutinating adhesion gene (saa) from piglets in Mizoram

Journal article

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Detection and molecular characterization of Shiga toxin producing Escherichia coli (STEC) autoagglutinating adhesion gene (saa) from piglets in Mizoram

J. L. Kataria, T. Dutta, P. Roychoudhury and Jully Gogoi Tiwari

Veterinary World, Vol.7(6), pp.373-376

2014

DOI: https://doi.org/10.14202/vetworld.2014.373-376

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Abstract

Agriculture

Agriculture, Dairy & Animal Science

Life Sciences & Biomedicine

Science & Technology

Veterinary Sciences

Aim: The study was carried out to detect and characterize the Shiga toxin producing Escherichia coli (STEC) auto agglutinating adhesion gene (saa) from Piglets in Mizoram. Materials and Methods: A total 100 fecal samples from 0-3 month old piglets with (60) or without (40) diarrhoea were collected from different parts of Mizoram and screened for the presence of E. coll. All the locus of enterocyte (LEE) negative STEC strains was tested for the presence saa gene by polymerase chain reaction (PCR). Results: In this study, a total of 254 E. colt isolated and identified. Altogether, 51 E. call were found to be positive for at least one virulence gene tested, of which 30 and 21 were classified as STEC and EPEC, respectively. A total of 4 E. coil isolates were found to he positive for saa gene, of which one was from diarrhoeic and three were from healthy piglets. Conclusions: STEC and EPEC are prevalent in pig population of Mizoram. Presence of saa gene positive E. coli in pigs in this region may pose a threat to public health.

Details

Title: Detection and molecular characterization of Shiga toxin producing Escherichia coli (STEC) autoagglutinating adhesion gene (saa) from piglets in Mizoram
Authors/Creators: J. L. Kataria - Central Agricultural University
T. Dutta - Central Agricultural University
P. Roychoudhury - Central Agricultural University
Jully Gogoi Tiwari
Publication Details: Veterinary World, Vol.7(6), pp.373-376
Publisher: Veterinary World
Identifiers: 991005703766107891
Murdoch Affiliation: School of Veterinary Medicine
Language: English
Resource Type: Journal article

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