Detection of Dientamoeba fragilis in animal faeces using species specific real time PCR assay

D. Chan; J. Barratt; T. Roberts; O. Phillips; J. Šlapeta; U. Ryan; D. Marriott; J. Harkness; J. Ellis; D. Stark

doi:10.1016/j.vetpar.2016.07.025

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Detection of Dientamoeba fragilis in animal faeces using species specific real time PCR assay

Journal article

Open access

Peer reviewed

Detection of Dientamoeba fragilis in animal faeces using species specific real time PCR assay

D. Chan, J. Barratt, T. Roberts, O. Phillips, J. Šlapeta, U. Ryan, D. Marriott, J. Harkness, J. Ellis and D. Stark

Veterinary Parasitology, Vol.227, pp.42-47

2016

DOI: https://doi.org/10.1016/j.vetpar.2016.07.025

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Abstract

Dientamoeba fragilis is a potentially pathogenic, enteric, protozoan parasite with a worldwide distribution. While clinical case reports and prevalence studies appear regularly in the scientific literature, little attention has been paid to this parasite’s biology, life cycle, host range, and possible transmission routes. Overall, these aspects of Dientamoeba biology remain poorly understood at best. In this study, a total of 420 animal samples, collected from Australia, were surveyed for the presence of Dientamoeba fragilis using PCR. Several PCR assays were evaluated for sensitivity and specificity. Two previously published PCR methods demonstrated cross reactivity with other trichomonads commonly found in animal samples. Only one assay exhibited excellent specificity. Using this assay D. fragilis was detected from one dog and one cat sample. This is the first report of D. fragilis from these animals and highlights the role companion animals may play in D. fragilis transmission. This study demonstrated that some published D. fragilis molecular assays cross react with other closely related trichomonads and consequently are not suitable for animal prevalence studies.

Details

Title: Detection of Dientamoeba fragilis in animal faeces using species specific real time PCR assay
Authors/Creators: D. Chan (Author/Creator) - University of Technology Sydney
J. Barratt (Author/Creator) - University of Technology Sydney
T. Roberts (Author/Creator) - St Vincent's Health
O. Phillips (Author/Creator) - St Vincent's Health
J. Šlapeta (Author/Creator) - The University of Sydney
U. Ryan (Author/Creator) - Murdoch University
D. Marriott (Author/Creator) - St Vincent's Health
J. Harkness (Author/Creator) - St Vincent's Health
J. Ellis (Author/Creator) - University of Technology Sydney
D. Stark (Author/Creator) - University of Technology Sydney
Publication Details: Veterinary Parasitology, Vol.227, pp.42-47
Publisher: Elsevier BV
Identifiers: 991005540359407891
Copyright: © 2016 Elsevier B.V.
Murdoch Affiliation: School of Veterinary and Life Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.246 Diarrheal Diseases; 1.246.985 Cryptosporidium
Web Of Science research areas: Parasitology; Veterinary Sciences
ESI research areas: Plant & Animal Science