Journal article
Detection of turnip crinkle virus on agarose gel electropherograms at the nanogram load level
Electrophoresis, Vol.9(7), pp.299-302
1988
Abstract
The previous conditions for the physical characterization of turnip crinkle virus (TCV) by quantitative agarose gel electrophoresis [1, 2] were limiting the method to the microgram load level and were therefore insufficiently sensitive to satisfy the need in many areas of virology for detection of viruses containing single-stranded RNA at the nanogram level. The present report remedies that defect by presenting a technique compatible with the nanogram load level of such viruses. The technique is based on a reduction of gel thickness and on the use of silver staining.
Details
- Title
- Detection of turnip crinkle virus on agarose gel electropherograms at the nanogram load level
- Authors/Creators
- L. Orbán (Author/Creator) - National Institute of Child HealthA. Chrambach (Author/Creator) - Health and Human Development (2HD) Research Network
- Publication Details
- Electrophoresis, Vol.9(7), pp.299-302
- Publisher
- John Wiley & Sons Ltd
- Identifiers
- 991005543855907891
- Copyright
- © 1988 VCH Verlagsgesellschaft mbH
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 2 Chemistry
- 2.166 Chromatography & Electrophoresis
- 2.166.730 Capillary Electrophoresis
- Web Of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical
- ESI research areas
- Chemistry