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Journal article
Determinants of disease penetrance in PRPF31-associated retinopathy
Genes, Vol.12(10), Article 1542
2021
Abstract
Retinitis pigmentosa 11 (RP11) is caused by dominant mutations in PRPF31, however a significant proportion of mutation carriers do not develop retinopathy. Here, we investigated the relationship between CNOT3 polymorphism, MSR1 repeat copy number and disease penetrance in RP11 patients and non-penetrant carriers (NPCs). We further characterized PRPF31 and CNOT3 expression in fibroblasts from eight RP11 patients and one NPC from a family carrying the c.1205C>T variant. Retinal organoids (ROs) and retinal pigment epithelium (RPE) were differentiated from induced pluripotent stem cells derived from RP11 patients, an NPC and a control subject. All RP11 patients were homozygous for the 3-copy MSR1 repeat in the PRPF31 promoter, while 3/5 NPCs carried a 4-copy MSR1 repeat. The CNOT3 rs4806718 genotype did not correlate with disease penetrance. PRFP31 expression declined with age in adult cadaveric retina. PRPF31 and CNOT3 expression was reduced in RP11 fibroblasts, RO and RPE compared with controls. Both RP11 and NPC RPE displayed shortened primary cilia compared with controls, however a subpopulation of cells with normal cilia lengths was present in NPC RPE monolayers. Our results indicate that RP11 non-penetrance is associated with the inheritance of a 4-copy MSR1 repeat, but not with CNOT3 polymorphisms.
Details
- Title
- Determinants of disease penetrance in PRPF31-associated retinopathy
- Authors/Creators
- S. McLenachan (Author/Creator) - The University of Western AustraliaD. Zhang (Author/Creator) - Lions Eye InstituteJ. Grainok (Author/Creator) - Murdoch UniversityX. Zhang (Author/Creator) - The University of Western AustraliaZ. Huang (Author/Creator) - The University of Western AustraliaS-C Chen (Author/Creator) - Lions Eye InstituteK. Zaw (Author/Creator) - Lions Eye InstituteA. Lima (Author/Creator) - Murdoch UniversityL. Jennings (Author/Creator) - Lions Eye InstituteD. Roshandel (Author/Creator) - The University of Western AustraliaS.Y. Moon (Author/Creator) - Lions Eye InstituteR.C. Heath Jeffery (Author/Creator)M.S. Attia (Author/Creator) - The University of Western AustraliaJ.A. Thompson (Author/Creator) - Sir Charles Gairdner HospitalT.M. Lamey (Author/Creator) - The University of Western AustraliaT.L. McLaren (Author/Creator) - The University of Western AustraliaJ. De Roach (Author/Creator) - The University of Western AustraliaS. Fletcher (Author/Creator) - Murdoch UniversityF.K. Chen (Author/Creator) - The University of Western Australia
- Publication Details
- Genes, Vol.12(10), Article 1542
- Publisher
- MDPI
- Identifiers
- 991005540054107891
- Copyright
- © 2021 by the authors
- Murdoch Affiliation
- Centre for Molecular Medicine and Innovative Therapeutics
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.36 Ophthalmology
- 1.36.212 Genetic Retinopathies
- Web Of Science research areas
- Genetics & Heredity
- ESI research areas
- Molecular Biology & Genetics