Determination of discriminatory power of genetic markers used for genotyping Giardia duodenalis

S. Siripattanapipong; S. Leelayoova; M. Mungthin; R.C.A. Thompson; P. Boontanom; W. Saksirisamphant; P. Tan-ariya

doi:10.1111/j.1439-0523.2011.01854.x

Back

Determination of discriminatory power of genetic markers used for genotyping Giardia duodenalis

Journal article

Open access

Peer reviewed

Determination of discriminatory power of genetic markers used for genotyping Giardia duodenalis

S. Siripattanapipong, S. Leelayoova, M. Mungthin, R.C.A. Thompson, P. Boontanom, W. Saksirisamphant and P. Tan-ariya

Southeast Asian Journal of Tropical Medicine and Public Health, Vol.42(4), pp.764-771

2011

DOI: https://doi.org/10.1111/j.1439-0523.2011.01854.x

Files and links (2)

pdf

discriminatory_power_of_genetic_markers.pdfDownload View

Published (Version of Record) Open Access

url

Link to Published Version *Subscription may be requiredView

Abstract

Small subunit ribosomal DNA (SSU-rDNA), glutamate dehydrogenase (gdh), beta-giardin, triosephosphate isomerase (tpi), and elongation factor 1-alpha (ef1-alpha) genes are useful genetic markers for genotypic analysis of the intestinal protozoan, Giardia duodenalis (syn. G. lamblia, G. intestinalis), the cause of enteric disease in humans. To quantitatively compare the discriminatory power of these loci, 43 fecal samples were collected from central, northern and eastern Thailand and G. duodenalis specimens were analyzed using PCR-based genotyping and subcloning methods. Approximately equal prevalence of assemblage A (21) and B (22) were present among these populations. Analysis of Simpson's index and Wallace coefficient values from assemblage B isolates together with the data obtained from Gen Bank showed that the combination of two loci provides a higher discrimination power for subgenotyping G. duodenalis than using any single locus.

Details

Title: Determination of discriminatory power of genetic markers used for genotyping Giardia duodenalis
Authors/Creators: S. Siripattanapipong (Author/Creator)
S. Leelayoova (Author/Creator)
M. Mungthin (Author/Creator)
R.C.A. Thompson (Author/Creator)
P. Boontanom (Author/Creator)
W. Saksirisamphant (Author/Creator)
P. Tan-ariya (Author/Creator)
Publication Details: Southeast Asian Journal of Tropical Medicine and Public Health, Vol.42(4), pp.764-771
Publisher: SEAMEO Regional Tropical Medicine and Public Health Network
Identifiers: 991005544325407891
Murdoch Affiliation: School of Veterinary and Biomedical Sciences
Language: English
Resource Type: Journal article
Publisher URL: http://www.tm.mahidol.ac.th/seameo/publication.htm

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites

Metrics

251 File views/ downloads

95 Record Views

15 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Industry collaboration; Domestic collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.97 Plant Pathology; 3.97.636 Fungal Plant Pathogens
Web Of Science research areas: Agronomy; Biotechnology & Applied Microbiology; Plant Sciences
ESI research areas: Plant & Animal Science