Development and evaluation of a rapid immunomagnetic bead assay for the detection of classical swine fever virus antigen

J.V. Conlan; S. Khounsy; S.D. Blacksell; C.J. Morrissy; C.R. Wilks; L.J. Gleeson

doi:10.1007/s11250-008-9279-2

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Development and evaluation of a rapid immunomagnetic bead assay for the detection of classical swine fever virus antigen

Journal article

Open access

Peer reviewed

Development and evaluation of a rapid immunomagnetic bead assay for the detection of classical swine fever virus antigen

J.V. Conlan, S. Khounsy, S.D. Blacksell, C.J. Morrissy, C.R. Wilks and L.J. Gleeson

Tropical Animal Health and Production, Vol.41(6), pp.913-920

2009

DOI: https://doi.org/10.1007/s11250-008-9279-2

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Abstract

Classical swine fever (CSF) is a highly contagious and severe viral disease of swine resulting in substantial production losses in different farming systems in many regions of the world. The accurate and rapid detection of CSF outbreaks is reliant on sensitive and specific laboratory testing and is a key component of disease control. Specific detection of CSF virus can be achieved by virus isolation in tissue culture, antigen capture or the detection of viral RNA using molecular techniques. In order to reduce the time taken to achieve a diagnostic result and simplify testing methods, an antigen capture ELISA using immunomagnetic beads (IMB) as the solid phase was developed and compared to a microplate-based antigen capture (AC)-ELISA. The IMB-ELISA has up to 64-fold greater analytical sensitivity than the AC-ELISA and initial estimates of diagnostic sensitivity and specificity are 100%. The IMB-ELISA has a highly robust, rapid and stable test format and is simpler to perform than the AC-ELISA. The IMB-ELISA has the added advantage that a result can be sensitively and specifically determined by eye, lending it to the possibility of adaptation to a near-to-field test with minimal equipment or expertise needed.

Details

Title: Development and evaluation of a rapid immunomagnetic bead assay for the detection of classical swine fever virus antigen
Authors/Creators: J.V. Conlan (Author/Creator) - Australian Centre for International Agricultural Research
S. Khounsy (Author/Creator) - Ministry of Agriculture and Forestry
S.D. Blacksell (Author/Creator) - Mahidol University
C.J. Morrissy (Author/Creator) - Australian Centre for Disease Preparedness
C.R. Wilks (Author/Creator) - The University of Melbourne
L.J. Gleeson (Author/Creator) - Australian Centre for Disease Preparedness
Publication Details: Tropical Animal Health and Production, Vol.41(6), pp.913-920
Publisher: Kluwer Academic Publishers
Identifiers: 991005541357207891
Copyright: © Springer Science + Business Media B.V. 2008
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.104 Virology - General; 1.104.1882 Livestock Viral Threats
Web Of Science research areas: Agriculture, Dairy & Animal Science; Veterinary Sciences
ESI research areas: Plant & Animal Science