Development of EST-PCR markers for Thinopyrum intermedium chromosome 2Ai#2 and their application in characterization of novel wheat-grass recombinants

M.J. Wang; Y. Zhang; Z.S. Lin; X.G. Ye; Y.P. Yuan; W. Ma; Z.Y. Xin

doi:10.1007/s00122-010-1394-6

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Development of EST-PCR markers for Thinopyrum intermedium chromosome 2Ai#2 and their application in characterization of novel wheat-grass recombinants

Journal article

Peer reviewed

Development of EST-PCR markers for Thinopyrum intermedium chromosome 2Ai#2 and their application in characterization of novel wheat-grass recombinants

M.J. Wang, Y. Zhang, Z.S. Lin, X.G. Ye, Y.P. Yuan, W. Ma and Z.Y. Xin

Theoretical and Applied Genetics, Vol.121(7), pp.1369-1380

2010

DOI: https://doi.org/10.1007/s00122-010-1394-6

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Abstract

A series of expressed sequence tags-derived polymerase chain reaction (EST-PCR) markers specific to chromosome 2Ai#2 from Thinopyrum intermedium were developed in this study using a new integrative approach. The target alien chromosome confers high resistance to barley yellow dwarf virus (BYDV), which is a severe virus disease in wheat. To generate markers evenly distributed on 2Ai#2, a total of 105 primer pairs were designed based on mapped ESTs from 8 bins of wheat chromosome 2B with intron-prediction by aligning ESTs with genomic sequences of the new model plant Brachypodium distachyon. Eight and seven polymorphic markers on the short arm and the long arm of chromosome 2Ai#2, respectively, were obtained with a polymorphism rate of 14.3%. These chromosome 2Ai#2-specific EST-PCR markers were then used in tracing and exploring the structural variation of the alien chromosome in the population derived from the immature embryo culture of the cross between N452, a 2Ai#2(2D) substitution line, and common wheat CB037. Two centric fusion of translocations involving 2Ai#2 short or long arm with wheat chromosome 2D and some new genetic stocks including telosomes with the alien chromosome short or long arm were identified in the SC3 generations, which provided basic materials to further study the mechanism of the BYDV resistance. BYDV tests in two field seasons suggest that the BYDV resistance was mainly conferred by the short arm, gene interaction on both arms of the alien chromosome was discussed.

Details

Title: Development of EST-PCR markers for Thinopyrum intermedium chromosome 2Ai#2 and their application in characterization of novel wheat-grass recombinants
Authors/Creators: M.J. Wang (Author/Creator) - Jilin Medical University
Y. Zhang (Author/Creator) - Inner Mongolia Agricultural University
Z.S. Lin (Author/Creator) - Civil Aviation Authority
X.G. Ye (Author/Creator) - Civil Aviation Authority
Y.P. Yuan (Author/Creator) - Jilin University
W. Ma (Author/Creator) - Murdoch University
Z.Y. Xin (Author/Creator) - Civil Aviation Authority
Publication Details: Theoretical and Applied Genetics, Vol.121(7), pp.1369-1380
Publisher: Springer Verlag
Identifiers: 991005541160507891
Murdoch Affiliation: State Agricultural Biotechnology Centre
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.96 QTL
Web Of Science research areas: Agronomy; Genetics & Heredity; Horticulture; Plant Sciences
ESI research areas: Agricultural Sciences