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Development of a GC–MS method for methamphetamine detection in Calliphora vomitoria L. (Diptera: Calliphoridae)
Journal article   Open access   Peer reviewed

Development of a GC–MS method for methamphetamine detection in Calliphora vomitoria L. (Diptera: Calliphoridae)

P.A. Magni, T. Pacini, M. Pazzi, M. Vincenti and I.R. Dadour
Forensic Science International, Vol.241, pp.96-101
2014
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Abstract

Entomotoxicology is the study of using insects for the detection of drugs and other chemical substances in decomposing tissues. One research aspect in particular is the effects of these substances on arthropod development and morphology, and their consequences on the post mortem interval estimation. Since methamphetamine (MA) is becoming commonplace as an illegal recreational drug, a GC-MS method for the detection of MA in Calliphora vomitoria L. (Diptera: Calliphoridae) was developed and validated. Furthermore, the effect of MA on the development, growth rates and survival of the blowfly was investigated. Larvae were reared on liver substrates homogeneously spiked with measured amounts of MA (5. ng/g and 10. ng/g) based on typical concentrations found in human tissue in cases of death caused by MA overdose. The experimental results demonstrated that (i) MA produced a significant increase in the developmental time from egg to adult in C. vomitoria, (ii) approximately 60% of larvae exposed to either dose of MA died during the pupation period and (iii) the resultant lengths of larvae and pupae were on average significantly larger than the controls.

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Domestic collaboration
International collaboration
Citation topics
3 Agriculture, Environment & Ecology
3.32 Entomology
3.32.1638 Forensic Entomology
Web Of Science research areas
Medicine, Legal
ESI research areas
Clinical Medicine
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