Journal article
Development of a duplex PCR assay for detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli in pig feces
Journal of Clinical Microbiology, Vol.41(7), pp.3372-3375
2003
Abstract
A duplex PCR (D-PCR) amplifying portions of the Brachyspira hyodysenteriae NADH oxidase gene and the B. pilosicoli 16S rRNA gene was developed and then tested on DNA extracted from 178 porcine fecal samples. The feces also underwent anaerobic culture and species-specific PCRs. Fecal extraction-D-PCR detected seven additional samples containing B. hyodysenteriae and five more containing B. pilosicoli.
Details
- Title
- Development of a duplex PCR assay for detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli in pig feces
- Authors/Creators
- T. La (Author/Creator) - Murdoch UniversityN.D. Phillips (Author/Creator) - Murdoch UniversityD.J. Hampson (Author/Creator) - Murdoch University
- Publication Details
- Journal of Clinical Microbiology, Vol.41(7), pp.3372-3375
- Publisher
- American Society for Microbiology
- Identifiers
- 991005541032107891
- Copyright
- © 2003, American Society for Microbiology
- Murdoch Affiliation
- School of Veterinary and Biomedical Sciences
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.248 Sexually Transmitted Infections
- 1.248.2445 Lawsonia Intracellularis
- Web Of Science research areas
- Microbiology
- ESI research areas
- Microbiology