Development of a modified selective medium to enhance the recovery rate of Brachyspira hyodysenteriae and other porcine intestinal spirochaetes from faeces

K. Lugsomya; P. Tummaruk; D.J. Hampson; N. Prapasarakul

doi:10.1111/j.1472-765X.2012.03213.x

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Development of a modified selective medium to enhance the recovery rate of Brachyspira hyodysenteriae and other porcine intestinal spirochaetes from faeces

Journal article

Peer reviewed

Development of a modified selective medium to enhance the recovery rate of Brachyspira hyodysenteriae and other porcine intestinal spirochaetes from faeces

K. Lugsomya, P. Tummaruk, D.J. Hampson and N. Prapasarakul

Letters in Applied Microbiology, Vol.54(4), pp.330-335

2012

DOI: https://doi.org/10.1111/j.1472-765X.2012.03213.x

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Abstract

Aims: The aim of this study was to develop a modified selective medium to improve the recovery rate of Brachyspira hyodysenteriae and other clinically significant intestinal spirochaetes from porcine faeces. Methods and Results: The susceptibility of five Brachyspira spp. type strains and five Thai field isolates of B. hyodysenteriae to the antimicrobials halquinol and flavomycin was determined by in vitro susceptibility tests in the agar dilution method, and optimal incorporation rates were confirmed by broth dilution. All the spirochaetes were susceptible to halquinol at ≤1μgml -1, while 16μgml -1 of flavomycin (F) allowed their growth, and therefore, only the latter was selected for further use. F and different combinations of colistin (C), spectinomycin (S) and rifampacin (R) were incorporated into pre-enrichment broths and/or agar plates, and growth of the spirochaetes from seeded faeces was determined. Two solid media were selected for further testing using faeces from 90 finishing pigs on 10 farms. A previously recommended method of pre-enrichment did not increase the recovery rate. The use of blood agar modified medium (BAM) containing F (16μgml -1), S (400μgml -1), R (30μgml -1) and colistin (C, 100Uml -1) (assigning as BAM-CSRF) reduced the growth of contaminating intestinal microbiota and resulted in a significantly higher rate of spirochaete recovery than the previous recommended medium. Conclusion: BAM-CSRF is a useful new selective medium for the isolation of B. hyodysenteriae and other intestinal spirochaetes from pig faeces. Significance and Impact of the Study: The new selective medium for isolating B. hyodysenteriae and other Brachyspira spp. from pig faeces will improve their recovery and subsequent disease diagnosis.

Details

Title: Development of a modified selective medium to enhance the recovery rate of Brachyspira hyodysenteriae and other porcine intestinal spirochaetes from faeces
Authors/Creators: K. Lugsomya (Author/Creator) - Chulalongkorn University
P. Tummaruk (Author/Creator) - Chulalongkorn University
D.J. Hampson (Author/Creator) - Murdoch University
N. Prapasarakul (Author/Creator) - Chulalongkorn University
Publication Details: Letters in Applied Microbiology, Vol.54(4), pp.330-335
Publisher: Blackwell Publishing
Identifiers: 991005543402407891
Copyright: © 2012 The Authors.
Murdoch Affiliation: Animal Research Institute; School of Veterinary and Biomedical Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.248 Sexually Transmitted Infections; 1.248.2445 Lawsonia Intracellularis
Web Of Science research areas: Biotechnology & Applied Microbiology; Microbiology
ESI research areas: Biology & Biochemistry