Development of a multilocus sequence tool for typing Cryptosporidium muris and Cryptosporidium andersoni

Y. Feng; W. Yang; U. Ryan; L. Zhang; M. Kvac; B. Koudela; D. Modry; N. Li; R. Fayer; L. Xiao

doi:10.1128/JCM.01329-10

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Development of a multilocus sequence tool for typing Cryptosporidium muris and Cryptosporidium andersoni

Journal article

Open access

Peer reviewed

Development of a multilocus sequence tool for typing Cryptosporidium muris and Cryptosporidium andersoni

Y. Feng, W. Yang, U. Ryan, L. Zhang, M. Kvac, B. Koudela, D. Modry, N. Li, R. Fayer and L. Xiao

Journal of Clinical Microbiology, Vol.49(1), pp.34-41

2011

DOI: https://doi.org/10.1128/JCM.01329-10

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Abstract

Although widely used for the characterization of the transmission of intestinal Cryptosporidium spp., genotyping tools are not available for C. muris and C. andersoni, two of the most common gastric Cryptosporidium spp. infecting mammals. In this study, we screened the C. muris whole-genome sequencing data for microsatellite and minisatellite sequences. Among the 13 potential loci (6 microsatellite and 7 minisatellite loci) evaluated by PCR and DNA sequencing, 4 were eventually chosen. DNA sequence analyses of 27 C. muris and 17 C. andersoni DNA preparations showed the presence of 5 to 10 subtypes of C. muris and 1 to 4 subtypes of C. andersoni at each locus. Altogether, 11 C. muris and 7 C. andersoni multilocus sequence typing (MLST) subtypes were detected among the 16 C. muris and 12 C. andersoni specimens successfully sequenced at all four loci. In all analyses, the C. muris isolate (TS03) that originated from an East African mole rat differed significantly from other C. muris isolates, approaching the extent of genetic differences between C. muris and C. andersoni. Thus, an MLST technique was developed for the highresolution typing of C. muris and C. andersoni. It should be useful for the characterization of the population genetics and transmission of gastric Cryptosporidium spp.

Details

Title: Development of a multilocus sequence tool for typing Cryptosporidium muris and Cryptosporidium andersoni
Authors/Creators: Y. Feng (Author/Creator)
W. Yang (Author/Creator)
U. Ryan (Author/Creator)
L. Zhang (Author/Creator)
M. Kvac (Author/Creator)
B. Koudela (Author/Creator)
D. Modry (Author/Creator)
N. Li (Author/Creator)
R. Fayer (Author/Creator)
L. Xiao (Author/Creator)
Publication Details: Journal of Clinical Microbiology, Vol.49(1), pp.34-41
Publisher: American Society for Microbiology
Identifiers: 991005542525307891
Copyright: © 2011, American Society for Microbiology
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.246 Diarrheal Diseases; 1.246.985 Cryptosporidium
Web Of Science research areas: Microbiology
ESI research areas: Microbiology