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Development of a multiplex, PCR-based genotyping assay for African and Asian elephants for forensic purposes
Journal article   Peer reviewed

Development of a multiplex, PCR-based genotyping assay for African and Asian elephants for forensic purposes

M.J. Potoczniak, M. Chermak, L. Quarino, S.S. Tobe and J. Conte
International Journal of Legal Medicine, Vol.134(1), pp.55-62
2020
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Abstract

Wildlife crimes and the threats they present to elephant populations raise the need to develop and implement DNA-based methodology as an aid for wildlife forensic investigations and conservation efforts. This study describes the development of a tetra-nucleotide repeat STR multiplex, genotyping assay that will identify Asian elephant (Elephas maximus) and African elephant (Loxodonta africana) DNA. The assay targets six tetra-nucleotide STRs and two sex-typing markers simultaneously in both genera of elephants, a first for elephant genotyping assays. The developed assay has potential application in wildlife investigations to associate a biological sample to a particular individual elephant and additionally in conservation science for population management.

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Collaboration types
International collaboration
Citation topics
3 Agriculture, Environment & Ecology
3.64 Phylogenetics & Genomics
3.64.71 Genetic Diversity
Web Of Science research areas
Medicine, Legal
ESI research areas
Clinical Medicine
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