Journal article
Development of a non-targeted metabolomics method to investigate urine in a rat model of polycystic kidney disease
Nephrology, Vol.17(2), pp.104-110
2012
Abstract
Aim: The purpose of this research was to use metabolomics to investigate the cystic phenotype in the Lewis polycystic kidney rat. Methods: Spot urine samples were collected from four male Lewis control and five male Lewis polycystic kidney rats aged 5 weeks, before kidney function was significantly impaired. Metabolites were extracted from urine and analysed using gas chromatography-mass spectrometry. Principal component analysis was used to determine key metabolites contributing to the variance observed between sample groups. Results: With the development of a metabolomics method to analyse Lewis and Lewis polycystic kidney rat urine, 2-ketoglutaric acid, allantoin, uric acid and hippuric acid were identified as potential biomarkers of cystic disease in the rat model. Conclusion: The findings of this study demonstrate the potential of metabolomics to further investigate kidney disease.
Details
- Title
- Development of a non-targeted metabolomics method to investigate urine in a rat model of polycystic kidney disease
- Authors/Creators
- H. Abbiss (Author/Creator) - Edith Cowan UniversityG.L. Maker (Author/Creator) - Murdoch UniversityJ. Gummer (Author/Creator)M. Sharman (Author/Creator) - Edith Cowan UniversityJ.K. Phillips (Author/Creator) - Australian School of Advanced MedicineM. Boyce (Author/Creator) - Schools of Natural SciencesR.D. Trengove (Author/Creator) - Murdoch University
- Publication Details
- Nephrology, Vol.17(2), pp.104-110
- Publisher
- Blackwell Publishing
- Identifiers
- 991005545421807891
- Copyright
- © Asian Pacific Society of Nephrology
- Murdoch Affiliation
- Separation Science and Metabolomics Laboratory; School of Veterinary and Biomedical Sciences
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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- Collaboration types
- Domestic collaboration
- Citation topics
- 2 Chemistry
- 2.211 Mass Spectrometry
- 2.211.990 Metabolomics
- Web Of Science research areas
- Urology & Nephrology
- ESI research areas
- Clinical Medicine