Journal article
Development of an Immunocapture-Based Polymeric Optical Fiber Sensor for Bacterial Detection in Water
Polymers, Vol.16(6), 861
2024
PMID: 38543466
Abstract
Conventional methods for pathogen detection in water rely on time-consuming enrichment steps followed by biochemical identification strategies, which require assay times ranging from 24 hours to a week. However, in recent years, significant efforts have been made to develop biosensing technologies enabling rapid and close-to-real-time detection of waterborne pathogens. In previous studies, we developed a plastic optical fiber (POF) immunosensor using an optoelectronic configuration consisting of a U-Shape probe connected to an LED and a photodetector. Bacterial detection was evaluated with the immunosensor immersed in a bacterial suspension in water with a known concentration. Here, we report on the sensitivity of a new optoelectronic configuration consisting of two POF U-shaped probes, one as the reference and the other as the immunosensor, for the detection of Escherichia coli. In addition, another methos of detection was tested where the sensors were calibrated in the air, before being immersed in a bacterial suspension and then read in the air. This modification improved sensor sensitivity and resulted in a faster detection time. After the immunocapture, the sensors were DAPI-stained and submitted to confocal microscopy. The histograms obtained confirmed that the responses of the immunosensors were due to the bacteria. This new sensor detected the presence of E. coli at 104 CFU/mL in less than 20 min. Currently, sub-20 min is faster than previous studies using fiber-optic based biosensors. We report on an inexpensive and faster detection technology when compared with conventional methods.
Details
- Title
- Development of an Immunocapture-Based Polymeric Optical Fiber Sensor for Bacterial Detection in Water
- Authors/Creators
- Rafaela Nascimento Lopes - Univ Fed Rio De Janeiro, Elect Engn Program, COPPE, BR-21941598 Rio De Janeiro, BrazilPaulo Henrique Silva Pinto - Univ Fed Rio De Janeiro, Elect Engn Program, COPPE, BR-21941598 Rio De Janeiro, BrazilJuan David Lopez Vargas - Univ Fed Rio De Janeiro, Nanotechnol Engn Program, COPPE, BR-21941598 Rio De Janeiro, BrazilAlex Dante - Univ Fed Rio De Janeiro, Elect Engn Program, COPPE, BR-21941598 Rio De Janeiro, BrazilAndrew Macrae - Univ Fed Rio De Janeiro, Programa Posgrad Biotecnol Vegetal & Bioproc, CCS, BR-21941598 Rio De Janeiro, BrazilRegina Celia Barros Allil - Univ Fed Rio De Janeiro, Elect Engn Program, COPPE, BR-21941598 Rio De Janeiro, BrazilMarcelo Martins Werneck - Univ Fed Rio De Janeiro, Elect Engn Program, COPPE, BR-21941598 Rio De Janeiro, Brazil
- Publication Details
- Polymers, Vol.16(6), 861
- Publisher
- Mdpi
- Number of pages
- 13
- Grant note
- Coordenao de Aperfeioamento de Pessoal de Nvel Superior-Brasil (CAPES); Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
- Identifiers
- 991005894542507891
- Copyright
- © 2024 by the authors.
- Murdoch Affiliation
- Food Futures Institute
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: SDGs in the Output
Metrics
1 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Citation topics
- 5 Physics
- 5.38 Optical Electronics & Engineering
- 5.38.232 Fiber Optic Sensors
- Web Of Science research areas
- Polymer Science
- ESI research areas
- Chemistry