Development of an immunoassay to measure progesterone using printed biosensors, and its application to the assessment of ovarian function in the numbat (Myrmecobius fasciatus)

W. Ditcham; C. Palmer; K. Warren; C. Monaghan; W Kappelle; P. Matson

doi:10.1080/15321810902782921

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Development of an immunoassay to measure progesterone using printed biosensors, and its application to the assessment of ovarian function in the numbat (Myrmecobius fasciatus)

Journal article

Peer reviewed

Development of an immunoassay to measure progesterone using printed biosensors, and its application to the assessment of ovarian function in the numbat (Myrmecobius fasciatus)

W. Ditcham, C. Palmer, K. Warren, C. Monaghan, W Kappelle and P. Matson

Journal of Immunoassay and Immunochemistry, Vol.30(2), pp.232-243

2009

DOI: https://doi.org/10.1080/15321810902782921

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Abstract

A biosensor system was developed to measure progesterone levels in the urine of female numbats (Myrmecobius fasciata) as an index of ovarian function. Screen printed sensors were coated with a monoclonal progesterone antibody, and incubated in a mixture of sample/standard and progesterone-3-CMO-horseradish peroxidase (HRP). The difference in potential between the working and reference electrode was measured, after exposure to an HRP substrate. EIA and biosensor standard curves showed parallelism, and the biosensor gave values similar (r=0.83) to the conventional EIA. Progesterone concentrations at different stages of the oestrus cycle were not significantly different to those obtained by EIA.

Details

Title: Development of an immunoassay to measure progesterone using printed biosensors, and its application to the assessment of ovarian function in the numbat (Myrmecobius fasciatus)
Authors/Creators: W. Ditcham (Author/Creator) - Murdoch University
C. Palmer (Author/Creator) - Murdoch University
K. Warren (Author/Creator) - Murdoch University
C. Monaghan (Author/Creator) - Murdoch University
W Kappelle (Author/Creator) - Murdoch University
P. Matson (Author/Creator) - Murdoch University
Publication Details: Journal of Immunoassay and Immunochemistry, Vol.30(2), pp.232-243
Publisher: Marcel Dekker Inc.
Identifiers: 991005542607107891
Copyright: (c) Taylor & Francis
Murdoch Affiliation: School of Veterinary and Biomedical Sciences
Language: English
Resource Type: Journal article

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Citation topics: 3 Agriculture, Environment & Ecology; 3.35 Zoology & Animal Ecology; 3.35.693 Primate Behavior
Web Of Science research areas: Biochemical Research Methods; Immunology; Medical Laboratory Technology
ESI research areas: Biology & Biochemistry