Journal article
Development of gene-specific markers for acid soil/aluminium tolerance in barley (Hordeum vulgare L.)
Molecular Breeding, Vol.32(1), pp.155-164
2013
Abstract
Acid soil/aluminium toxicity is one of the major constraints on barley production around the world. Genetic improvement is the best solution and molecular-marker-assisted selection has proved to be an efficient tool for developing barley cultivars with acid soil/aluminium tolerance. In this study, barley variety Svanhals-introduced from CYMMIT (International Maize and Wheat Improvement Center)-was identified as acid soil/aluminium tolerant and the tolerance was mapped to chromosome 4H in 119 doubled haploid (DH) lines from a cross of Hamelin/Svanhals. The HvMATE gene, encoding an aluminium-activated citrate transporter, was selected as a candidate gene and gene-specific molecular markers were developed to detect acid soil/aluminium tolerance based on the polymerase chain reaction. Sequence analysis of the HvMATE gene identified a 21-bp indel (insertion-deletion) between the tolerant and sensitive cultivars. The new marker was further mapped to the QTL (quantitative trait loci) region on chromosome 4H for acid soil tolerance and accounted for 66.9 % of phenotypic variation in the DH population. Furthermore, the polymorphism was confirmed in other tolerant varieties which have been widely used as a source of acid soil tolerance in Australian barley breeding programs. The new gene-specific molecular marker provides an effective and simple molecular tool for selecting the acid soil tolerance gene from multiple tolerance sources.
Details
- Title
- Development of gene-specific markers for acid soil/aluminium tolerance in barley (Hordeum vulgare L.)
- Authors/Creators
- M. Bian (Author/Creator) - Huazhong Agricultural UniversityI. Waters (Author/Creator) - Agriculture and FoodS. Broughton (Author/Creator) - Agriculture and FoodX-Q Zhang (Author/Creator) - Murdoch UniversityM. Zhou (Author/Creator) - University of TasmaniaR. Lance (Author/Creator) - Agriculture and FoodD. Sun (Author/Creator) - Huazhong Agricultural UniversityC. Li (Author/Creator) - Murdoch University
- Publication Details
- Molecular Breeding, Vol.32(1), pp.155-164
- Publisher
- Kluwer Academic Publishers
- Identifiers
- 991005545462807891
- Copyright
- © 2013 Springer Science+Business Media Dordrecht
- Murdoch Affiliation
- State Agricultural Biotechnology Centre
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.45 Soil Science
- 3.45.473 Soil Phosphorus Dynamics
- Web Of Science research areas
- Agronomy
- Genetics & Heredity
- Horticulture
- Plant Sciences
- ESI research areas
- Plant & Animal Science