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Development of reference ranges for plasma total cholinesterase and brain acetylcholinesterase activity in free ranging Carnaby's black cockatoos (Calyptorhynchus latirostris)
Journal article   Open access   Peer reviewed

Development of reference ranges for plasma total cholinesterase and brain acetylcholinesterase activity in free ranging Carnaby's black cockatoos (Calyptorhynchus latirostris)

R.J. Vaughan-Higgins, S.D. Vitali, A. Reiss, S. Besier, T. Hollingsworth and G. Smith
Journal of Wildlife Diseases, Vol.52(3), pp.592-598
19/05/2016
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Abstract

Published avian reference ranges for plasma cholinesterase (ChE) and brain acetylcholinesterase (AChE) are numerous. However, a consistently reported recommendation is the need for species- and laboratory-specific reference ranges because of variables, including assay methods, sample storage conditions, season, and bird sex, age, and physiologic status. We developed normal reference ranges for brain AChE and plasma total ChE (tChE) activity for Carnaby's Black-Cockatoos (Calyptorhynchus latirostris) using a standardized protocol (substrate acetylthiocholine at 25 C). We report reference ranges for brain AChE (19-41 μmol/min per g, mean 21±6.38) and plasma tChE (0.41-0.53 μmol/min per mL, mean 0.47±0.11) (n=15). This information will be of use in the ongoing field investigation of a paresis-paralysis syndrome in the endangered Carnaby's Black-Cockatoos, suspected to be associated with exposure to anticholinesterase compounds and add to the paucity of reference ranges for plasma tChE and brain AChE in Australian psittacine birds.

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Citation topics
3 Agriculture, Environment & Ecology
3.60 Herbicides, Pesticides & Ground Poisoning
3.60.548 Cholinesterase Inhibition
Web Of Science research areas
Veterinary Sciences
ESI research areas
Plant & Animal Science
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