Journal article
Development of robust PCR-based DNA markers for each homoeo-allele of granule-bound starch synthase and their application in wheat breeding programs
Australian Journal of Agricultural Research, Vol.52(12), pp.1409-1416
2001
Abstract
The absence of expression of the granule-bound starch synthase I (GBSSI) allele from chromosome 4A of wheat is associated with improved starch quality for making Udon noodles. Several PCR-based methods for the analysis of GBSS alleles have been developed for application in wheat. A widely applied approach has involved a simple PCR followed by electrophoretic separation of DNA products on agarose gels. The PCR amplifies one band from each of the loci on chromosomes 4A (Wx-B1), 7A (Wx-A1), and 7D (Wx-D1), and the band from the Wx-B1 locus is diagnostic for the occurrence of the null Wx-B1 allele that is associated with improved starch quality. The reliable detection of the null Wx-B1 allele has been important in identifying wheat breeding lines. Allele-specific PCR has also been used to successfully detect the occurrence of the null Wx-B1 allele. In the present paper the various protocols were evaluated by testing a segregating double haploid population from a cross between Cranbrook and Halberd and the tests gave good agreement in different laboratories. The application of the DNAbased tests applied in wheat breeding programs provides one of the first examples of a molecular marker selection for a grain quality trait being successfully applied in an Australian wheat breeding program.
Details
- Title
- Development of robust PCR-based DNA markers for each homoeo-allele of granule-bound starch synthase and their application in wheat breeding programs
- Authors/Creators
- A. McLauchlan (Author/Creator)F.C. Ogbonnaya (Author/Creator)B. Hollingsworth (Author/Creator)M. Carter (Author/Creator)K.R. Gale (Author/Creator)R.J. Henry (Author/Creator)T.A. Holton (Author/Creator)M.K. Morell (Author/Creator)L.R. Rampling (Author/Creator)P.J. Sharp (Author/Creator)M.R. Shariflou (Author/Creator)M.G.K. Jones (Author/Creator)R. Appels (Author/Creator)
- Publication Details
- Australian Journal of Agricultural Research, Vol.52(12), pp.1409-1416
- Publisher
- CSIRO
- Identifiers
- 991005541242207891
- Copyright
- © 2001 CSIRO
- Murdoch Affiliation
- State Agricultural Biotechnology Centre
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.85 Food Science & Technology
- 3.85.99 Cereal Starch Properties
- Web Of Science research areas
- Agriculture, Multidisciplinary
- ESI research areas
- Agricultural Sciences