Development of sheep preimplantation embryos in media supplemented with glucose and acetate

J.G.E. Thompson; G.A.J. Parton; G.W. Cruickshank; J.F. Smith; R.G. Wales

doi:10.1016/0093-691X(89)90322-1

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Development of sheep preimplantation embryos in media supplemented with glucose and acetate

Journal article

Peer reviewed

Development of sheep preimplantation embryos in media supplemented with glucose and acetate

J.G.E. Thompson, G.A.J. Parton, G.W. Cruickshank, J.F. Smith and R.G. Wales

Theriogenology, Vol.32(2), pp.323-330

1989

DOI: https://doi.org/10.1016/0093-691X(89)90322-1

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Abstract

Two experiments were conducted to examine the effect of supplemental glucose (G; 1.5 mM) and/or acetate (A; 0.5 mM) on the development of early sheep embryos to blastocysts when cultured in vitro in glucose-free synthetic oviductal fluid (SOF) + sheep serum or bovine serum albumin (BSA). In Experiment 1, 2- to 4-cell, 8- to 16-cell and >16-cell embryos were cultured in SOF, SOF+G, SOF+A or SOF+G+A. All media were supplemented with 10% sheep serum. In addition, embryos were cultured in either microdrops under polysiloxane oil or in multiwell dishes. Overall, development to the blastocyst stage was 3%, 30% and 68% for 2- to 4-cell, 8- to 16-cell and >16-cell stages, respectively, suggesting that an 8-cell developmental block existed under our culture conditions. Glucose supplementation had little effect on embryo development, and no overall effect was observed from the addition of acetate. In Experiment 2, 8- to 16-cell embryos were cultured in SOF or SOF+G, both supplemented with BSA. Development to the blastocyst stage was 25% and 18%, respectively. The results show that the presence of glucose or acetate did little to enhance embryonic development in our incubation systems. Further work is required to evaluate fully the energy requirements for development of the early sheep embryo.

Details

Title: Development of sheep preimplantation embryos in media supplemented with glucose and acetate
Authors/Creators: J.G.E. Thompson (Author/Creator)
G.A.J. Parton (Author/Creator)
G.W. Cruickshank (Author/Creator)
J.F. Smith (Author/Creator)
R.G. Wales (Author/Creator)
Publication Details: Theriogenology, Vol.32(2), pp.323-330
Publisher: Elsevier BV
Identifiers: 991005542535607891
Murdoch Affiliation: School of Veterinary Studies
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.81 Reproductive Biology; 1.81.339 Embryo Development
Web Of Science research areas: Reproductive Biology; Veterinary Sciences
ESI research areas: Plant & Animal Science