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Development of simple sequence repeat markers for Botryosphaeria spp. with Fusicoccum anamorphs
Journal article   Peer reviewed

Development of simple sequence repeat markers for Botryosphaeria spp. with Fusicoccum anamorphs

B. Slippers, T. Burgess, B.D. Wingfield, P.W. Crous, T.A. Coutinho and M.J. Wingfield
Molecular Ecology Notes, Vol.4(4), pp.675-677
2004
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Abstract

We report the development of eight sets of microsatellite markers for the ascomycete fungus and tree pathogen, Botryosphaeria parva. The primers were identified after cloning and sequencing of fragments amplified using simple sequence repeat (SSR) primers. Genome walking was used to determine unknown sequences on either side of new SSRs. The primers were tested and proved useful in nine other Botryosphaeria species that all have Fusicoccum anamorphs, similar to B. parva.

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