Journal article
Diagnostic performance of a commercial immunoblot assay for myositis antibody testing
Pathology, Vol.48(4), pp.363-366
2016
Abstract
The objective of this study was to establish a population based reference range for a commercial immunoblot assay detecting myositis specific autoantibodies (MSAs) and myositis associated autoantibodies (MAAs), and to assess the diagnostic performance of this reference range against the manufacturer's recommended ranges in a myositis patient cohort.
A total of 124 patients from a myositis cohort and 197 healthy controls were serologically assessed using a commercial immunoblot containing eleven autoantigens (Jo-1, EJ, OJ, PL7, PL12, Mi-2, SRP, Ku, PMScl75, PMScl100 and Ro52) according to the manufacturer's instructions.
Use of the manufacturer's reference ranges resulted in detection of MSAs in 19.4% of myositis patients and 9.1% of controls; MAAs were detected in 41.1% of myositis patients and 14.2% of controls. Reference values derived from the healthy control population resulted in significant differences in cut-off values for some autoantibodies, particularly Ro52 and PMScl75. Use of local reference ranges reduced detection of MSAs to 16.9% of myositis patients and 3% of healthy controls, with MAAs 23.4% of patients and 2% of healthy controls.
Application of population based reference ranges resulted in significant differences in detection of MSAs and MAAs compared to the manufacturer's recommended ranges. Cut-off levels should be assessed to ensure suitability for the population tested.
Details
- Title
- Diagnostic performance of a commercial immunoblot assay for myositis antibody testing
- Authors/Creators
- C. Bundell (Author/Creator) - Pathwest Laboratory MedicineA. Rojana-udomsart (Author/Creator) - The University of Western AustraliaF. Mastaglia (Author/Creator) - Murdoch UniversityP. Hollingsworth (Author/Creator) - The University of Western AustraliaA. McLean-Tooke (Author/Creator) - Pathwest Laboratory Medicine
- Publication Details
- Pathology, Vol.48(4), pp.363-366
- Publisher
- Elsevier Inc.
- Identifiers
- 991005540951407891
- Copyright
- © 2016 Published by Elsevier Inc.
- Murdoch Affiliation
- Institute for Immunology and Infectious Diseases
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.106 Rheumatology
- 1.106.1684 Dermatomyositis
- Web Of Science research areas
- Pathology
- ESI research areas
- Clinical Medicine