Journal article
Dichromate digestion-spectrophotometric procedure for determination of soil microbial biomass carbon in association with fumigation-extraction
Communications in Soil Science and Plant Analysis, Vol.42(22), pp.2824-2834
2011
Abstract
A dichromate digestion and spectrophotometric procedure is proposed for estimating soil microbial biomass carbon (C) in association with fumigation-extraction. The recommended procedure uses a volume (1.6 ml) of 0.5 M potassium sulfate (K2SO4) soil extracts and oxidant solution (dichromate-sulfuric acid, 2.4 mL), mixed with a volume (4 mL) for digestion at 140 °C for 30 min. The digested solution is then directly read for absorbance at 350 nm using a spectrophotometer, and the C in the digested soil extracts is measured against glucose standards. The KEC (indicating the extractable part of microbial biomass C after fumigation) value is estimated as 0.33 for the proposed method. There are good correlations between soil microbial biomass C measured by the proposed method, the dichromate digestion titration, and oven oxidation by total organic C (TOC) analytical method. This method is a simple, rapid, and economical procedure associated with fumigation-extraction for biomass C analysis.
Details
- Title
- Dichromate digestion-spectrophotometric procedure for determination of soil microbial biomass carbon in association with fumigation-extraction
- Authors/Creators
- Y. Cai (Author/Creator)C. Peng (Author/Creator)S. Qiu (Author/Creator)Y. Li (Author/Creator)
- Publication Details
- Communications in Soil Science and Plant Analysis, Vol.42(22), pp.2824-2834
- Publisher
- Marcel Dekker Inc.
- Number of pages
- 11
- Identifiers
- 991005541058807891
- Copyright
- © Taylor & Francis Group, LLC.
- Murdoch Affiliation
- School of Environmental Science
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.45 Soil Science
- 3.45.112 Soil Carbon Dynamics
- Web Of Science research areas
- Agronomy
- Chemistry, Analytical
- Plant Sciences
- Soil Science
- ESI research areas
- Agricultural Sciences