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Differences in antigen expression within and between 10 isolates of Giardia duodenalis
Journal article   Peer reviewed

Differences in antigen expression within and between 10 isolates of Giardia duodenalis

R. Hopkins, R.C.A. Thompson, R.P. Hobbs, A.J. Lymbery, N. Villa and T.M. Smithyman
Acta Tropica, Vol.54(2), pp.117-124
1993
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Abstract

In this study indirect immunofluorescence was performed on both live and fixed trophozoites to determine the level of variability in surface antigen expression between 14 Giardia duodenalis isolates, using a monoclonal antibody raised against the Portland I isolate (ATCC No. 30888). Subsets of antigen positive cells were detected in 13 isolates ranging in number from <1% to 50% of the total population. The differences in antigen expression between 10 uncloned isolates did not correlate with genetic differences determined using isoenzyme analysis. Examination of four clones of the Portland I isolate showed that all of the progeny expressed surface antigen at significantly different levels to the parent.

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Citation topics
1 Clinical & Life Sciences
1.246 Diarrheal Diseases
1.246.985 Cryptosporidium
Web Of Science research areas
Parasitology
Tropical Medicine
ESI research areas
Clinical Medicine
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