Journal article
Different exosomal microRNA profile in Aquaporin-4 Antibody Positive Neuromyelitis Optica Spectrum Disorders
Frontiers in Immunology, Vol.11, Art. 1064
2020
Abstract
Neuromyelitis optica spectrum disorders (NMOSD) and multiple sclerosis (MS) are inflammatory demyelinating diseases of the central nervous system. Exosomal microRNAs (miRNAs) are emerging biomarkers for demyelinating diseases. In this study, 52 aquaporin-4 antibody serum-positive NMOSD patients, 18 relapsing-remitting multiple sclerosis (RRMS) patients and 17 healthy controls (HCs) were included for the next-generation sequencing (NGS). To validate the NGS results, the valuable miRNAs were selected for validation by real-time quantitative polymerase chain reaction in another cohort of patients, comprising 31 NMOSD patients and 14 HCs. In addition, these miRNAs were also validated in a longitudinal study. NGS data revealed the exosomal miRNAs profile in NMOSD patients was different from HCs. Among those potential exosomal miRNAs which can distinguish NMOSD status, hsa-miR-122-3p and hsa-miR-200a-5p were the most abundant miRNAs. In addition, hsa-miR-122-3p and hsa-miR-200a-5p were significantly upregulated in the serum exosome of relapsing NMOSD compared with that in remitting NMOSD. Hsa-miR-122-3p and hsa-miR-200a-5p had positive correlations with disease severity in NMOSD patients. Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that the MAPK, Wnt and Ras signaling pathways were enriched. Further biological function analysis demonstrated that these two miRNAs might be involved in the immunoregulation of NMOSD pathogenesis. Our results indicated that miRNAs delivered by exosomes could be applied as potential biomarkers for NMOSD.
Details
- Title
- Different exosomal microRNA profile in Aquaporin-4 Antibody Positive Neuromyelitis Optica Spectrum Disorders
- Authors/Creators
- C. Chen (Author/Creator) - Third Affiliated Hospital of Sun Yat-sen UniversityY. Wu (Author/Creator) - Third Affiliated Hospital of Sun Yat-sen UniversityM. Li (Author/Creator) - The First People's Hospital of ZhaoqingC. Cui (Author/Creator) - Third Affiliated Hospital of Sun Yat-sen UniversityY. Zhao (Author/Creator) - Third Affiliated Hospital of Sun Yat-sen UniversityX. Sun (Author/Creator)Y. Wang (Author/Creator) - Third Affiliated Hospital of Sun Yat-sen UniversityC. Liu (Author/Creator) - Third Affiliated Hospital of Sun Yat-sen UniversityH. Wu (Author/Creator) - Third Affiliated Hospital of Sun Yat-sen UniversityX. Zhong (Author/Creator) - Third Affiliated Hospital of Sun Yat-sen UniversityA.G. Kermode (Author/Creator) - Third Affiliated Hospital of Sun Yat-sen UniversityL. Peng (Author/Creator) - Third Affiliated Hospital of Sun Yat-sen UniversityW. Qiu (Author/Creator) - Third Affiliated Hospital of Sun Yat-sen University
- Publication Details
- Frontiers in Immunology, Vol.11, Art. 1064
- Publisher
- Frontiers Media
- Identifiers
- 991005543226007891
- Copyright
- © 2020 The Authors
- Murdoch Affiliation
- Institute for Immunology and Infectious Diseases
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
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- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.196 Micro & Long Noncoding RNA
- 1.196.68 MicroRNA in Cancer
- Web Of Science research areas
- Immunology
- ESI research areas
- Immunology