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Journal article
Differential acquisition of amino acid and peptide enantiomers within the soil microbial community and its implications for carbon and nitrogen cycling in soil
Soil biology & biochemistry, Vol.88, pp.83-89
2015
Abstract
l-isomeric amino acids and oligopeptides are thought to represent a key nitrogen (N) source for plants and soil microorganisms, bypassing the need to take up inorganic N, whilst self-cycling of d-enantiomers within peptidoglycan-containing bacteria may provide a further short circuit within the N cycle. Here we use stable isotope profiling (SIP) to identify the fate of organic N within soil microbial communities. We followed the incorporation of 13C-labelled d- or l-labelled amino acids/peptides into phospholipid fatty acids (PLFAs). l-alanine and its peptides were taken up more rapidly than d-enantiomers by Gram-positive bacteria with 13C incorporation being predominantly into anteiso- and iso-fatty acids typically associated with Gram-positive bacteria. d-enantiomer uptake was found not to differ significantly between the microbial groups, providing little support for the view that soil bacteria may self-cycle d-forms of amino acids and peptides. There was no consistent association between peptide chain length and incorporation. The concentrations of l- and d-isomeric amino acids in soil solution were 866 nM and 72 nM, respectively. We conclude that Gram-positive bacteria appear to be the primary competitors for l-enantiomeric forms of amino acids and their peptides, but that both d- and l-enantiomers are available N and C sources for bacteria and fungi.
Details
- Title
- Differential acquisition of amino acid and peptide enantiomers within the soil microbial community and its implications for carbon and nitrogen cycling in soil
- Authors/Creators
- R.C.I. Broughton - British Antarctic SurveyK.K. Newsham - British Antarctic SurveyP.W. Hill - Bangor UniversityA. Stott - CEH Lancaster Environment Centre, Library Avenue, Bailrigg, Lancaster LA1 4AP, UKD.L. Jones - Bangor University
- Publication Details
- Soil biology & biochemistry, Vol.88, pp.83-89
- Publisher
- Elsevier Ltd
- Identifiers
- 991005560342907891
- Copyright
- © 2015 The Authors.
- Murdoch Affiliation
- Centre for Sustainable Farming Systems
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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InCites Highlights
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- Collaboration types
- Domestic collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.45 Soil Science
- 3.45.112 Soil Carbon Dynamics
- Web Of Science research areas
- Soil Science
- ESI research areas
- Agricultural Sciences