Journal article
Differential anti-APOBEC3G activity of HIV-1 Vif proteins derived from different subtypes
Journal of Biological Chemistry, Vol.285(46), pp.35350-35358
2010
Abstract
Antiretroviral cytidine deaminase APOBEC3G, which is abundantly expressed in peripheral blood lymphocytes and macrophages, strongly protects these cells against HIV-1 infection. The HIV-1 Vif protein overcomes this antiviral effect by enhancing proteasome-mediated APOBEC3G degradation and is key for maintaining viral infectivity. The 579-bp-long vif gene displays high genetic diversity among HIV-1 subtypes. Therefore, it is intriguing to address whether Vif proteins derived from different subtypes differ in their viral defense activity against APOBEC3G. Expression plasmids encoding Vif proteins derived from subtypes A, B, C, CRF01-AE, and CRF02-AG isolates were created, and their anti-APOBEC3G activities were compared. Viruses produced from cells expressing APOBEC3G and Vif proteins from different subtypes showed relatively different viral infectivities. Notably, subtype C-derived Vif proteins tested had the highest activity against APOBEC3G that was ascribed to its increased binding activity, for which the N-terminal domain of the Vif protein sequences was responsible. These results suggest that the biological differences of Vif proteins belonging to different subtypes might affect viral fitness and quasispecies in vivo.
Details
- Title
- Differential anti-APOBEC3G activity of HIV-1 Vif proteins derived from different subtypes
- Authors/Creators
- Y. Iwabu (Author/Creator) - National Institute of Infectious DiseasesM. Kinomoto (Author/Creator) - National Institute of Infectious DiseasesM. Tatsumi (Author/Creator) - National Institute of Infectious DiseasesH. Fujita (Author/Creator) - Kyushu UniversityM. Shimura (Author/Creator) - the Department of Intractable Diseases, International Medical Center of Japan, Tokyo 162-8655, Japan, andY. Tanaka (Author/Creator) - Kyushu UniversityY. Ishizaka (Author/Creator) - the Department of Intractable Diseases, International Medical Center of Japan, Tokyo 162-8655, Japan, andD. Nolan (Author/Creator) - Royal Perth HospitalS. Mallal (Author/Creator) - Royal Perth HospitalT. Sata (Author/Creator) - National Institute of Infectious DiseasesK. Tokunaga (Author/Creator) - National Institute of Infectious Diseases
- Publication Details
- Journal of Biological Chemistry, Vol.285(46), pp.35350-35358
- Publisher
- American Society for Biochemistry and Molecular Biology Inc.
- Identifiers
- 991005543233607891
- Copyright
- © 2010 by The American Society for Biochemistry and Molecular Biology
- Murdoch Affiliation
- Centre for Clinical Immunology and Biomedical Statistics
- Language
- English
- Resource Type
- Journal article
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- Domestic collaboration
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- Citation topics
- 1 Clinical & Life Sciences
- 1.66 HIV
- 1.66.1243 Integrase
- Web Of Science research areas
- Biochemistry & Molecular Biology
- ESI research areas
- Biology & Biochemistry