Differential gene expression in the Meristem and during early fruit growth of Pisum sativum L. identifies potential targets for breeding

Annu Smitha Ninan; Anish Shah; Jiancheng Song; Paula E Jameson

doi:10.3390/ijms18020428

Back

Differential gene expression in the Meristem and during early fruit growth of Pisum sativum L. identifies potential targets for breeding

Journal article

Open access

Peer reviewed

Differential gene expression in the Meristem and during early fruit growth of Pisum sativum L. identifies potential targets for breeding

Annu Smitha Ninan, Anish Shah, Jiancheng Song and Paula E Jameson

International journal of molecular sciences, Vol.18(2), Art. 428

2017

DOI: https://doi.org/10.3390/ijms18020428

PMCID: PMC5343962

PMID: 28212324

Files and links (1)

pdf

Published2.35 MBDownload View

CC BY V4.0, Open Access

Abstract

Cluster Analysis

Fruit - genetics

Fruit - growth & development

Gene Expression Profiling

Gene Expression Regulation, Plant

Meristem - genetics

Peas - genetics

Phylogeny

Plant Breeding

Plant Shoots - genetics

Transcriptome

Plant biology

Plant cell and molecular biology

For successful molecular breeding it is important to identify targets to the gene family level, and in the specific species of interest, in this case Pisum sativum L. The cytokinins have been identified as a key breeding target due to their influence on plant architecture, and on seed size and sink activity. We focused on the cytokinin biosynthetic gene family (the IPTs) and the gene family key to the destruction of cytokinins (the CKXs), as well as other gene families potentially affected by changing cytokinin levels. These included key meristem genes (WUS and BAM1) and the transporter gene families, sucrose transporters (SUTs) and amino acid permeases (AAPs). We used reverse transcription quantitative PCR (RT-qPCR) to monitor gene expression in the vegetative meristem and in pre- and post-fertilisation young pea fruits. PsWUS expression was specific to the shoot apical meristem while PsBAM1 was highly expressed in the shoot apical meristem (SAM) but was also expressed at a low level in the young fruit. Differential expression was shown between genes and within gene families for IPT, CKX, SUT, and AAP. PsCKX7 showed strong gene family member-specific expression in the SAM, and was also expressed in young pea fruits. We suggest that PsCKX7 is a potential target for downregulation via molecular breeding or gene editing.

Details

Title: Differential gene expression in the Meristem and during early fruit growth of Pisum sativum L. identifies potential targets for breeding
Authors/Creators: Annu Smitha Ninan - School of Biological Sciences, University of Canterbury, Christchurch 8140, New Zealand. annu.ninan@canterbury.ac.nz
Anish Shah
Jiancheng Song - School of Life Sciences, Yantai University, Yantai 264005, China. jcsong88@yahoo.com
Paula E Jameson - School of Biological Sciences, University of Canterbury, Christchurch 8140, New Zealand. paula.jameson@canterbury.ac.nz
Publication Details: International journal of molecular sciences, Vol.18(2), Art. 428
Publisher: MDPI
Identifiers: 991005565170007891
Murdoch Affiliation: School of Agricultural Sciences
Language: English
Resource Type: Journal article

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

#12 Responsible Consumption & Production

Metrics

7 File views/ downloads

70 Record Views

13 Times Cited - Web of Science

See more details

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.159 Arabidopsis
Web Of Science research areas: Biochemistry & Molecular Biology; Chemistry, Multidisciplinary
ESI research areas: Chemistry