Journal article
Differentiating phosphate-dependent and phosphate-independent systemic phosphate-starvation response networks in Arabidopsis thaliana through the application of phosphite
Journal of Experimental Botany, Vol.66(9), pp.2501-2514
2015
Abstract
Phosphite is a less oxidized form of phosphorus than phosphate. Phosphite is considered to be taken up by the plant through phosphate transporters. It can mimic phosphate to some extent, but it is not metabolized into organophosphates. Phosphite could therefore interfere with phosphorus signalling networks. Typical physiological and transcriptional responses to low phosphate availability were investigated and the short-term kinetics of their reversion by phosphite, compared with phosphate, were determined in both roots and shoots of Arabidopsis thaliana. Phosphite treatment resulted in a strong growth arrest. It mimicked phosphate in causing a reduction in leaf anthocyanins and in the expression of a subset of the phosphate-starvation-responsive genes. However, the kinetics of the response were slower than for phosphate, which may be due to discrimination against phosphite by phosphate transporters PHT1;8 and PHT1;9 causing delayed shoot accumulation of phosphite. Transcripts encoding PHT1;7, lipid-remodelling enzymes such as SQD2, and phosphocholine-producing NMT3 were highly responsive to phosphite, suggesting their regulation by a direct phosphate-sensing network. Genes encoding components associated with the 'PHO regulon' in plants, such as At4, IPS1, and PHO1;H1, generally responded more slowly to phosphite than to phosphate, except for SPX1 in roots and MIR399d in shoots. Two uncharacterized phosphate-responsive E3 ligase genes, PUB35 and C3HC4, were also highly phosphite responsive. These results show that phosphite is a valuable tool to identify network components directly responsive to phosphate.
Details
- Title
- Differentiating phosphate-dependent and phosphate-independent systemic phosphate-starvation response networks in Arabidopsis thaliana through the application of phosphite
- Authors/Creators
- R. Jost (Author/Creator) - The University of Western AustraliaM. Pharmawati (Author/Creator) - The University of Western AustraliaH.R. Lapis-Gaza (Author/Creator) - The University of Western AustraliaC. Rossig (Author/Creator) - The University of Western AustraliaO. Berkowitz (Author/Creator) - The University of Western AustraliaH. Lambers (Author/Creator) - The University of Western AustraliaP.M. Finnegan (Author/Creator) - The University of Western Australia
- Publication Details
- Journal of Experimental Botany, Vol.66(9), pp.2501-2514
- Publisher
- Oxford University Press
- Identifiers
- 991005545205107891
- Copyright
- © The Author 2015.
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
53 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.45 Soil Science
- 3.45.473 Soil Phosphorus Dynamics
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science