Differentiation between human and animal isolates of Cryptosporidium parvum using rDNA sequencing and direct PCR analysis

U.M. Morgan; C.C. Constantine; D.A. Forbes; R.C.A. Thompson

doi:10.2307/3284275

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Differentiation between human and animal isolates of Cryptosporidium parvum using rDNA sequencing and direct PCR analysis

Journal article

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Peer reviewed

Differentiation between human and animal isolates of Cryptosporidium parvum using rDNA sequencing and direct PCR analysis

U.M. Morgan, C.C. Constantine, D.A. Forbes and R.C.A. Thompson

Journal of Parasitology, Vol.83(5), pp.825-830

1997

DOI: https://doi.org/10.2307/3284275

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Abstract

Sequence analysis of a polymerase chain reaction (PCR)-amplified 298-bp region of the Cryptosporidium parvum 18S rRNA gene was carried out on 10 human and 9 animal isolates. Eight of the 9 animal isolates and 3 human isolates displayed the recognition sequence TATATTT, whereas 7/10 human isolates exhibited the recognition sequence TTTTTTTTTTT. Sequence analysis of the ninth animal isolate, which was recovered from a Koala, revealed this isolate to be different from both human and animal isolates. The AT richness of the rDNA recognition sequences rendered them unsuitable for primer design and therefore a diagnostic randomly amplified polymorphic DNA fragment previously developed in our laboratory was also sequenced. Analysis of 2 human and 2 animal isolates again revealed distinct differences between animal and human isolates. On the basis of this sequence information, diagnostic primers were designed that could directly differentiate between animal and human isolates on the basis of the size of the PCR product. The ability to differentiate directly between human and animal isolates has important implications for studies of the transmission and zoonotic potential of this organism. These results also raise further doubts about the uniformity of the species C. parvum.

Details

Title: Differentiation between human and animal isolates of Cryptosporidium parvum using rDNA sequencing and direct PCR analysis
Authors/Creators: U.M. Morgan (Author/Creator) - Murdoch University
C.C. Constantine (Author/Creator)
D.A. Forbes (Author/Creator) - School of Paediatrics and Child Health
R.C.A. Thompson (Author/Creator)
Publication Details: Journal of Parasitology, Vol.83(5), pp.825-830
Publisher: American Society of Parasitologists
Identifiers: 991005541320607891
Copyright: © 1997 American Society of Parasitologists
Murdoch Affiliation: School of Veterinary and Biomedical Sciences
Language: English
Resource Type: Journal article

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Citation topics: 1 Clinical & Life Sciences; 1.246 Diarrheal Diseases; 1.246.985 Cryptosporidium
Web Of Science research areas: Parasitology
ESI research areas: Microbiology