Journal article
Differing requirements for flavonoids during the formation of lateral roots, nodules and root knot nematode galls in Medicago truncatula
New Phytologist, Vol.183(1), pp.167-179
2009
Abstract
In this study, we tested whether the organogenesis of symbiotic root nodules, lateral roots and root galls induced by parasitic root knot nematodes (Meloidogyne javanica) was regulated by the presence of flavonoids in the roots of Medicago truncatula. Flavonoids accumulate in all three types of root organ, and have been hypothesized previously to be required for secondary root organogenesis because of their potential role as auxin transport regulators. • Using RNA interference to silence the flavonoid biosynthetic pathway in M. truncatula, we generated transformed flavonoid-deficient hairy roots which were used to study flavonoid accumulation, cell division and organogenesis of nodules, lateral roots and root galls. • Flavonoid-deficient roots did not form nodules, as demonstrated previously, but showed altered root growth in response to rhizobia. By contrast, flavonoid-deficient roots showed no difference in the number of lateral roots and root galls. Galls on flavonoid-deficient roots formed normal giant cells, but were shorter, and were characterized by reduced numbers of dividing pericycle cells. • We rejected the hypothesis that flavonoids are required as general regulators of the organogenesis of secondary root organs, but flavonoids appear to be necessary for nodulation. Possible reasons for this difference in the requirement for flavonoids are discussed.
Details
- Title
- Differing requirements for flavonoids during the formation of lateral roots, nodules and root knot nematode galls in Medicago truncatula
- Authors/Creators
- A.P. Wasson (Author/Creator)K. Ramsay (Author/Creator)M.G.K. Jones (Author/Creator)U. Mathesius (Author/Creator)
- Publication Details
- New Phytologist, Vol.183(1), pp.167-179
- Publisher
- Blackwell Publishing
- Identifiers
- 991005545389307891
- Copyright
- © New Phytologist (2009).
- Murdoch Affiliation
- School of Biological Sciences and Biotechnology
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.97 Plant Pathology
- 3.97.1108 Nematode Management
- Web Of Science research areas
- Plant Sciences
- ESI research areas
- Plant & Animal Science