Journal article
Direct application of plasmid DNA containing type I interferon transgenes to vaginal mucosa inhibits HSV-2 mediated mortality
Biological Procedures Online, Vol.8(1), pp.55-62
2006
Abstract
The application of naked DNA containing type I interferon (IFN) transgenes is a promising potential therapeutic approach for controlling chronic viral infections. Herein, we detail the application of this approach that has been extensively used to restrain ocular HSV-1 infection, for antagonizing vaginal HSV-2 infection. We show that application of IFN-α1, -α 5, and -β transgenes to vaginal mouse lumen 24 hours prior to HSV-2 infection reduces HSV-2 mediated mortality by 2.5 to 3-fold. However, other type I IFN transgenes (IFN- α 4, -α 5, -α 6, and -α 9) are non effectual against HSV-2. We further show that the efficacy of IFN-1 transgene treatment is independent of CD4+ T lymphocytes. However, in mice depleted of CD8+ T lymphocytes, the ability of IFN-α 1 transgene treatment to antagonize HSV-2 was lost.
Details
- Title
- Direct application of plasmid DNA containing type I interferon transgenes to vaginal mucosa inhibits HSV-2 mediated mortality
- Authors/Creators
- B.A. Austin (Author/Creator)C.M. James-Berry (Author/Creator)P. Härle (Author/Creator)D.J.J. Carr (Author/Creator)
- Publication Details
- Biological Procedures Online, Vol.8(1), pp.55-62
- Publisher
- Springer
- Identifiers
- 991005544897807891
- Copyright
- 2006 The Authors
- Murdoch Affiliation
- School of Veterinary and Biomedical Sciences
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.161 Virology - Identification & Sequencing
- 1.161.315 Herpesvirus Dynamics
- Web Of Science research areas
- Biochemical Research Methods
- ESI research areas
- Biology & Biochemistry