Journal article
Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential
Parasitology, Vol.134(3), pp.359-367
03/2007
Abstract
In vitro propagation followed by PCR, and a PCR-based method capable of the direct detection of Blastocystis in faeces were utilized to detect Blastocystis from various hosts in Australia, including primates and their handlers from the Perth Zoo. In addition, Blastocystis isolates from dogs and humans living in a localized endemic community in Thailand were also characterized genetically. PCR-based detection directly from faeces was shown to be more sensitive compared with in vitro culture for the detection of Blastocystis. Moreover, phylogenetic analysis of Blastocystis isolates amplified utilizing in vitro techniques prior to PCR revealed that this method favoured the preferential amplification of Blastocystis subtype 5 over subtype 1. This study is the first to provide molecular-based evidence supporting the zoonotic potential of Blastocystis in dogs, possums and primates in a natural setting.
Details
- Title
- Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential
- Authors/Creators
- U. Parkar (Author/Creator) - Murdoch UniversityR.J. Traub (Author/Creator) - Murdoch UniversityS. Kumar (Author/Creator) - University of MalayaM. Mungthin (Author/Creator) - Phramongkutklao College of MedicineS. Vitali (Author/Creator) - Perth Zoo, South Perth, Western Australia 6151, AustraliaS. Leelayoova (Author/Creator) - Phramongkutklao College of MedicineK. Morris (Author/Creator) - Department of Environment, Land, Water and PlanningR.C.A. Thompson (Author/Creator) - Murdoch University
- Publication Details
- Parasitology, Vol.134(3), pp.359-367
- Publisher
- Cambridge University Press
- Identifiers
- 991005541351207891
- Copyright
- © 2006 Cambridge University Press.
- Murdoch Affiliation
- School of Veterinary and Biomedical Sciences
- Language
- English
- Resource Type
- Journal article
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