Journal article
Discontinuous buffer systems optimized for the agarose gel electrophoresis of subcellular particles
Electrophoresis, Vol.9(4), pp.167-171
1988
Abstract
Discontinuous buffer systems operative between pH 5.7 and 7.4, 0 °C, were generated, which are characterized by more rapidly displaced moving boundaries than applied previously. These allow one to resolve subcellular particles relatively rapidly and at relatively low agarose gel concentrations. A commercial mixture of DNA restriction fragments pre-stained with ethidium bromide was found to be a suitable tracking dye for these boundaries.
Details
- Title
- Discontinuous buffer systems optimized for the agarose gel electrophoresis of subcellular particles
- Authors/Creators
- L. Orbán (Author/Creator) - National Institute of Child HealthE. Hahn (Author/Creator) - Health and Human Development (2HD) Research NetworkA. Chrambach (Author/Creator) - Health and Human Development (2HD) Research Network
- Publication Details
- Electrophoresis, Vol.9(4), pp.167-171
- Publisher
- John Wiley & Sons Ltd
- Identifiers
- 991005541044407891
- Copyright
- © 1988 VCH Verlagsgesellschaft mbH
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 2 Chemistry
- 2.166 Chromatography & Electrophoresis
- 2.166.730 Capillary Electrophoresis
- Web Of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical
- ESI research areas
- Chemistry