Journal article
Discovery of genomic regions and candidate genes controlling shelling percentage using QTL ‐seq approach in cultivated peanut ( Arachis hypogaea L.)
Plant Biotechnology Journal, Vol.17(7), pp.1248-1260
2019
Abstract
Cultivated peanut (Arachis hypogaea L.) is an important grain legume providing high‐quality cooking oil, rich proteins and other nutrients. Shelling percentage (SP) is the 2nd most important agronomic trait after pod yield and this trait significantly affects the economic value of peanut in the market. Deployment of diagnostic markers through genomics‐assisted breeding (GAB) can accelerate the process of developing improved varieties with enhanced SP. In this context, we deployed the QTL‐seq approach to identify genomic regions and candidate genes controlling SP in a recombinant inbred line population (Yuanza 9102 × Xuzhou 68‐4). Four libraries (two parents and two extreme bulks) were constructed and sequenced, generating 456.89–790.32 million reads and achieving 91.85%–93.18% genome coverage and 14.04–21.37 mean read depth. Comprehensive analysis of two sets of data (Yuanza 9102/two bulks and Xuzhou 68‐4/two bulks) using the QTL‐seq pipeline resulted in discovery of two overlapped genomic regions (2.75 Mb on A09 and 1.1 Mb on B02). Nine candidate genes affected by 10 SNPs with non‐synonymous effects or in UTRs were identified in these regions for SP. Cost‐effective KASP (Kompetitive Allele‐Specific PCR) markers were developed for one SNP from A09 and three SNPs from B02 chromosome. Genotyping of the mapping population with these newly developed KASP markers confirmed the major control and stable expressions of these genomic regions across five environments. The identified candidate genomic regions and genes for SP further provide opportunity for gene cloning and deployment of diagnostic markers in molecular breeding for achieving high SP in improved varieties.
Details
- Title
- Discovery of genomic regions and candidate genes controlling shelling percentage using QTL ‐seq approach in cultivated peanut ( Arachis hypogaea L.)
- Authors/Creators
- H. Luo (Author/Creator) - Oil Crops Research InstituteM.K. Pandey (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsA.W. Khan (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsJ. Guo (Author/Creator) - Oil Crops Research InstituteB. Wu (Author/Creator) - Oil Crops Research InstituteY. Cai (Author/Creator) - Oil Crops Research InstituteL. Huang (Author/Creator) - Oil Crops Research InstituteX. Zhou (Author/Creator) - Oil Crops Research InstituteY. Chen (Author/Creator) - Oil Crops Research InstituteW. Chen (Author/Creator) - Oil Crops Research InstituteN. Liu (Author/Creator) - Oil Crops Research InstituteY. Lei (Author/Creator) - Oil Crops Research InstituteB. Liao (Author/Creator) - Oil Crops Research InstituteR.K. Varshney (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsH. Jiang (Author/Creator) - Oil Crops Research Institute
- Publication Details
- Plant Biotechnology Journal, Vol.17(7), pp.1248-1260
- Publisher
- Blackwell Publishing Inc.
- Identifiers
- 991005541311007891
- Copyright
- © 2018 The Authors.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.96 QTL
- Web Of Science research areas
- Biotechnology & Applied Microbiology
- Plant Sciences
- ESI research areas
- Plant & Animal Science