Discovery of novel Bmy1 alleles increasing β-amylase activity in Chinese landraces and Tibetan wild barley for improvement of malting quality via MAS

X. Gong; S. Westcott; X-Q Zhang; G. Yan; R. Lance; G. Zhang; D. Sun; C. Li

doi:10.1371/journal.pone.0072875

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Discovery of novel Bmy1 alleles increasing β-amylase activity in Chinese landraces and Tibetan wild barley for improvement of malting quality via MAS

Journal article

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Discovery of novel Bmy1 alleles increasing β-amylase activity in Chinese landraces and Tibetan wild barley for improvement of malting quality via MAS

X. Gong, S. Westcott, X-Q Zhang, G. Yan, R. Lance, G. Zhang, D. Sun and C. Li

PLoS ONE, Vol.8(9), e72875

2013

DOI: https://doi.org/10.1371/journal.pone.0072875

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Abstract

China has a large barley germplasm collection which has not been well characterized and is therefore underutilized. The Bmy1 locus encoding the β-amylase enzyme on chromosome 4H has been well characterized in the worldwide barley germplasm collections due to its importance in the malting and brewing industry. The Bmy1 locus was chosen as an indicator to understand genetic potential for improvement of malting quality in Chinese landraces and Tibetan wild barley. The genetic diversity of 91 barley accessions was assessed using allele specific Multiplex-ready molecular markers. Eight accessions were further sequenced, based on the Multiplex-ready marker diversity for Bmy1 in the germplasm. Six of the eight accessions clustered together in a unique group, and showed similarities to 'Haruna Nijo', wild barley accession PI296896 and 'Ashqelon'. Sequence comparisons with the known Bmy1 alleles identified not only the existing 13 amino acid substitutions, but also a new substitution positioned at A387T from a Chinese landrace W127, which has the highest β-amylase activity. Two new alleles/haplotypes namely Bmy1-Sd1c and Bmy1-Sd5 were designated based on different amino acid combinations. We identified new amino acid combination of C115, D165, V233, S347 and V430 in the germplasm. The broad variation in both β-amylase activity and amino acid composition provides novel alleles for the improvement of malting quality for different brewing styles, which indicates the high potential value of the Chinese landraces and Tibetan wild barley.

Details

Title: Discovery of novel Bmy1 alleles increasing β-amylase activity in Chinese landraces and Tibetan wild barley for improvement of malting quality via MAS
Authors/Creators: X. Gong (Author/Creator) - Huazhong Agricultural University
S. Westcott (Author/Creator) - Agriculture and Food
X-Q Zhang (Author/Creator) - The University of Western Australia
G. Yan (Author/Creator) - The University of Western Australia
R. Lance (Author/Creator) - Agriculture and Food
G. Zhang (Author/Creator) - Zhejiang University
D. Sun (Author/Creator) - Huazhong Agricultural University
C. Li (Author/Creator) - Murdoch University
Publication Details: PLoS ONE, Vol.8(9), e72875
Publisher: Public Library of Science
Identifiers: 991005540090607891
Copyright: © 2013 Gong et al.
Murdoch Affiliation: State Agricultural Biotechnology Centre
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.85 Food Science & Technology; 3.85.99 Cereal Starch Properties
Web Of Science research areas: Plant Sciences
ESI research areas: Plant & Animal Science