Discovery of novel neutral glycosphingolipids in cereal crops: rapid profiling using reversed-phased HPLC-ESI-QqTOF with parallel reaction monitoring

Dingyi Yu; Berin A Boughton; Thusitha W T Rupasinghe; Camilla B Hill; Cornelia Herrfurth; Patricia Scholz; Ivo Feussner; Ute Roessner

doi:10.1038/s41598-023-49981-7

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Discovery of novel neutral glycosphingolipids in cereal crops: rapid profiling using reversed-phased HPLC-ESI-QqTOF with parallel reaction monitoring

Journal article

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Discovery of novel neutral glycosphingolipids in cereal crops: rapid profiling using reversed-phased HPLC-ESI-QqTOF with parallel reaction monitoring

Dingyi Yu, Berin A Boughton, Thusitha W T Rupasinghe, Camilla B Hill, Cornelia Herrfurth, Patricia Scholz, Ivo Feussner and Ute Roessner

Scientific reports, Vol.13(1), 22560

2023

DOI: https://doi.org/10.1038/s41598-023-49981-7

PMID: 38110595

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Abstract

Chromatography, High Pressure Liquid - methods

Edible Grain

Fatty Acids

Neutral Glycosphingolipids

Spectrometry, Mass, Electrospray Ionization - methods

Sphingolipids

Tandem Mass Spectrometry - methods

This study explores the sphingolipid class of oligohexosylceramides (OHCs), a rarely studied group, in barley (Hordeum vulgare L.) through a new lipidomics approach. Profiling identified 45 OHCs in barley (Hordeum vulgare L.), elucidating their fatty acid (FA), long-chain base (LCB) and sugar residue compositions; and was accomplished by monophasic extraction followed by reverse-phased high performance liquid chromatography electrospray ionisation quadrupole-time-of-flight tandem mass spectrometry (HPLC-ESI-QqTOF-MS/MS) employing parallel reaction monitoring (PRM). Results revealed unknown ceramide species and highlighted distinctive FA and LCB compositions when compared to other sphingolipid classes. Structurally, the OHCs featured predominantly trihydroxy LCBs associated with hydroxylated FAs and oligohexosyl residues consisting of two-five glucose units in a linear 1 → 4 linkage. A survey found OHCs in tissues of major cereal crops while noting their absence in conventional dicot model plants. This study found salinity stress had only minor effects on the OHC profile in barley roots, leaving questions about their precise functions in plant biology unanswered.

Details

Title: Discovery of novel neutral glycosphingolipids in cereal crops: rapid profiling using reversed-phased HPLC-ESI-QqTOF with parallel reaction monitoring
Authors/Creators: Dingyi Yu - The University of Melbourne
Berin A Boughton - Department of Animal, Plant and Soil Sciences, La Trobe Institute for Sustainable Agriculture and Food, La Trobe University, Bundoora, VIC, 3083, Australia. b.boughton@latrobe.edu.au
Thusitha W T Rupasinghe - AbSciex, 2 Gilda Court, Mulgrave, VIC, 3170, Australia
Camilla B Hill - Murdoch University
Cornelia Herrfurth - University of Göttingen
Patricia Scholz - Laboratoire Reproduction et Développement des Plantes
Ivo Feussner - Plant (United States)
Ute Roessner - Australian National University
Publication Details: Scientific reports, Vol.13(1), 22560
Publisher: Springer Nature
Grant note: FT130100326 / Australian Research Council Dyson Fellowship / University of Melbourne
Identifiers: 991005623667607891
Murdoch Affiliation: College of Environmental and Life Sciences; State Agricultural Biotechnology Centre; Western Barley Genetics Alliance
Language: English
Resource Type: Journal article

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