Discovery of two novel and adjacent QTLs on chromosome B02 controlling resistance against bacterial wilt in peanut variety Zhonghua 6

H. Luo; M.K. Pandey; Y. Zhi; H. Zhang; S. Xu; J. Guo; B. Wu; H. Chen; X. Ren; X. Zhou; Y. Chen; W. Chen; L. Huang; N. Liu; H.K. Sudini; R.K. Varshney; Y. Lei; B. Liao; H. Jiang

doi:10.1007/s00122-020-03537-9

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Discovery of two novel and adjacent QTLs on chromosome B02 controlling resistance against bacterial wilt in peanut variety Zhonghua 6

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Discovery of two novel and adjacent QTLs on chromosome B02 controlling resistance against bacterial wilt in peanut variety Zhonghua 6

H. Luo, M.K. Pandey, Y. Zhi, H. Zhang, S. Xu, J. Guo, B. Wu, H. Chen, X. Ren, X. Zhou, …

Theoretical and Applied Genetics, Vol.133(4), pp.1133-1148

2020

DOI: https://doi.org/10.1007/s00122-020-03537-9

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Abstract

Peanut (Arachis hypogaea) is an important oilseed crop worldwide. Utilization of genetic resistance is the most economic and effective approach to control bacterial wilt, one of the most devastating plant diseases, in peanut production. To accelerate the genetic improvement of bacterial wilt resistance (BWR) in peanut breeding programs, quantitative trait locus (QTL) mapping has been conducted for two resistant varieties. In this context, we deployed linkage mapping as well as sequencing-based mapping approach, QTL-seq, to identify genomic regions and candidate genes for BWR in another highly resistant variety Zhonghua 6. The recombination inbred line population (268 progenies) from the cross Xuhua 13 × Zhonghua 6 was used in BWR evaluation across five environments. QTL mapping using both SSR- and SNP-based genetic maps identified a stable QTL (qBWRB02-1) on chromosome B02 with 37.79–78.86% phenotypic variation explained (PVE) across five environments. The QTL-seq facilitated further dissection of qBWRB02-1 into two adjacent genomic regions, qBWRB02-1-1 (2.81–4.24 Mb) and qBWRB02-1-2 (6.54–8.75 Mb). Mapping of newly developed Kompetitive allele-specific PCR (KASP) markers on the genetic map confirmed their stable expressions across five environments. The effects of qBWRB02-1-1 (49.43–68.86% PVE) were much higher than qBWRB02-1-2 (3.96–6.48% PVE) and other previously reported QTLs. Nineteen putative candidate genes affected by 49 non-synonymous SNPs were identified for qBWRB02-1-1, and ten of them were predicted to code for disease resistance proteins. The major and stable QTL qBWRB02-1-1 and validated KASP markers could be deployed in genomics-assisted breeding (GAB) to develop improved peanut varieties with enhanced BWR

Details

Title: Discovery of two novel and adjacent QTLs on chromosome B02 controlling resistance against bacterial wilt in peanut variety Zhonghua 6
Authors/Creators: H. Luo (Author/Creator) - Oil Crops Research Institute
M.K. Pandey (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
Y. Zhi (Author/Creator) - Angel Yeast Co., Ltd, Yichang, 443003, Hubei, China.
H. Zhang (Author/Creator) - Oil Crops Research Institute
S. Xu (Author/Creator) - Oil Crops Research Institute
J. Guo (Author/Creator) - Oil Crops Research Institute
B. Wu (Author/Creator) - Oil Crops Research Institute
H. Chen (Author/Creator) - Oil Crops Research Institute
X. Ren (Author/Creator) - Oil Crops Research Institute
X. Zhou (Author/Creator) - Oil Crops Research Institute
Y. Chen (Author/Creator) - Oil Crops Research Institute
W. Chen (Author/Creator) - Oil Crops Research Institute
L. Huang (Author/Creator) - Oil Crops Research Institute
N. Liu (Author/Creator) - Oil Crops Research Institute
H.K. Sudini (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
R.K. Varshney (Author/Creator) - International Crops Research Institute for the Semi-Arid Tropics
Y. Lei (Author/Creator) - Oil Crops Research Institute
B. Liao (Author/Creator) - Oil Crops Research Institute
H. Jiang (Author/Creator) - Oil Crops Research Institute
Publication Details: Theoretical and Applied Genetics, Vol.133(4), pp.1133-1148
Publisher: Springer Verlag
Identifiers: 991005545131707891
Copyright: © 2020 The Authors.
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.96 QTL
Web Of Science research areas: Agronomy; Genetics & Heredity; Horticulture; Plant Sciences
ESI research areas: Agricultural Sciences