Journal article
Dissecting genomic hotspots underlying seed protein, oil, and sucrose content in an interspecific mapping population of soybean using high-density linkage mapping
Plant Biotechnology Journal, Vol.16(11), pp.1939-1953
2018
Abstract
The cultivated [Glycine max (L) Merr.] and wild [Glycine soja Siebold & Zucc.] soybean species comprise wide variation in seed composition traits. Compared to wild soybean, cultivated soybean contains low protein, high oil, and high sucrose. In this study, an interspecific population was derived from a cross between G. max (Williams 82) and G. soja (PI 483460B). This recombinant inbred line (RIL) population of 188 lines was sequenced at 0.3× depth. Based on 91 342 single nucleotide polymorphisms (SNPs), recombination events in RILs were defined, and a high‐resolution bin map was developed (4070 bins). In addition to bin mapping, quantitative trait loci (QTL) analysis for protein, oil, and sucrose was performed using 3343 polymorphic SNPs (3K‐SNP), derived from Illumina Infinium BeadChip sequencing platform. The QTL regions from both platforms were compared, and a significant concordance was observed between bin and 3K‐SNP markers. Importantly, the bin map derived from next‐generation sequencing technology enhanced mapping resolution (from 1325 to 50 Kb). A total of five, nine, and four QTLs were identified for protein, oil, and sucrose content, respectively, and some of the QTLs coincided with soybean domestication‐related genomic loci. The major QTL for protein and oil were mapped on Chr. 20 (qPro_20) and suggested negative correlation between oil and protein. In terms of sucrose content, a novel and major QTL were identified on Chr. 8 (qSuc_08) and harbours putative genes involved in sugar transport. In addition, genome‐wide association using 91 342 SNPs confirmed the genomic loci derived from QTL mapping. A QTL‐based haplotype using whole‐genome resequencing of 106 diverse soybean lines identified unique allelic variation in wild soybean that could be utilized to widen the genetic base in cultivated soybean.
Details
- Title
- Dissecting genomic hotspots underlying seed protein, oil, and sucrose content in an interspecific mapping population of soybean using high-density linkage mapping
- Authors/Creators
- G. Patil (Author/Creator) - University of MissouriT.D. Vuong (Author/Creator) - University of MissouriS. Kale (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsB. Valliyodan (Author/Creator) - University of MissouriR. Deshmukh (Author/Creator) - Université LavalC. Zhu (Author/Creator) - University of MissouriX. Wu (Author/Creator) - Crop Science Division Bayer CropScience Morrisville NC USAY. Bai (Author/Creator) - Dow AgroSciencesD. Yungbluth (Author/Creator) - University of MissouriF. Lu (Author/Creator) - Dow AgroSciencesS. Kumpatla (Author/Creator) - Dow AgroSciencesJ.G. Shannon (Author/Creator) - University of MissouriR.K. Varshney (Author/Creator) - International Crops Research Institute for the Semi-Arid TropicsH.T. Nguyen (Author/Creator) - University of Missouri
- Publication Details
- Plant Biotechnology Journal, Vol.16(11), pp.1939-1953
- Publisher
- Blackwell Publishing Inc.
- Identifiers
- 991005543748707891
- Copyright
- © 2018 The Authors.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Industry collaboration
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.96 QTL
- Web Of Science research areas
- Biotechnology & Applied Microbiology
- Plant Sciences
- ESI research areas
- Plant & Animal Science