Journal article
Dissecting the role of G-protein signalling in primary metabolism in the wheat pathogen Stagonospora nodorum
Microbiology, Vol.159(9), pp.1972-1985
2013
Abstract
Mutants of the wheat pathogenic fungus Stagonospora nodorum lacking G-protein subunits display a variety of phenotypes including melanization defects, primary metabolic changes and a decreased ability to sporulate. To better understand the causes of these phenotypes, Stagonospora nodorum strains lacking a Gα, Gβ or Gγ subunit were compared to a wild-type strain using metabolomics. Agar plate growth at 22 °C revealed a number of fundamental metabolic changes and highlighted the influential role of these proteins in glucose utilization. A further characterization of the mutants was undertaken during prolonged storage at 4 °C, conditions known to induce sporulation in these sporulation-deficient signalling mutants. The abundance of several compounds positively correlated with the onset of sporulation including the dissacharide trehalose, the tryptophan degradation product tryptamine and the secondary metabolite alternariol; metabolites all previously associated with sporulation. Several other compounds decreased or were absent during sporulation. The levels of one such compound (Unknown_35.27_2194_319) decreased from being one of the more abundant compounds to absence during pycnidial maturation. This study has shed light on the role of G-protein subunits in primary metabolism during vegetative growth and exploited the cold-induced sporulation phenomenon in these mutants to identify some key metabolic changes that occur during asexual reproduction.
Details
- Title
- Dissecting the role of G-protein signalling in primary metabolism in the wheat pathogen Stagonospora nodorum
- Authors/Creators
- J.P.A. Gummer (Author/Creator) - Murdoch UniversityR.D. Trengove (Author/Creator) - Murdoch UniversityR.P. Oliver (Author/Creator) - Curtin UniversityP.S. Solomon (Author/Creator) - Australian National University
- Publication Details
- Microbiology, Vol.159(9), pp.1972-1985
- Publisher
- Society for General Microbiology
- Identifiers
- 991005540228507891
- Copyright
- © 2013 SGM.
- Murdoch Affiliation
- Separation Science and Metabolomics Laboratory
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.148 Medical Mycology
- 1.148.240 Saccharomyces Cerevisiae
- Web Of Science research areas
- Microbiology
- ESI research areas
- Microbiology