Dissecting the telomere region of barley chromosome 5HL using rice genomic sequences as references: new markers for tracking a complex region in breeding

X-Q Zhang; C. Li; J. Panozzo; S. Westcott; G. Zhang; A. Tay; R. Appels; M. Jones; R. Lance

doi:10.1007/s11032-010-9408-2

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Dissecting the telomere region of barley chromosome 5HL using rice genomic sequences as references: new markers for tracking a complex region in breeding

Journal article

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Peer reviewed

Dissecting the telomere region of barley chromosome 5HL using rice genomic sequences as references: new markers for tracking a complex region in breeding

X-Q Zhang, C. Li, J. Panozzo, S. Westcott, G. Zhang, A. Tay, R. Appels, M. Jones and R. Lance

Molecular Breeding, Vol.27, pp.1-9

2011

DOI: https://doi.org/10.1007/s11032-010-9408-2

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Abstract

The terminal region of barley chromosome 5HL controls malt extract, diastatic power, free amino acid nitrogen, alpha-amylase activity, seed dormancy and pre-harvest sprouting. Comparative analysis of the barley and rice maps has established that the terminal region of barley chromosome 5HL is syntenic to rice chromosome 3L near the telomere end. The rice BAC (Bacterial Artificial Chromosome) sequences covering the region of chromosome 3L were used to search barley expressed sequenced tags database. Thirty-three genes were amplified by PCR (polymerase chain reaction) with the primers designed from barley ESTs (expressed sequence tag). Comparison of the sequences of the PCR generated DNA fragments revealed polymorphisms including single nucleotide polymorphism (SNP), insertions or deletions between the barley varieties. Seven new PCR based molecular markers were developed and mapped within 10 cM in three doubled haploid barley populations (Stirling × Harrington, Baudin × AC Metcalfe and Chebec × Harrington). The mapped genes maintain the micro-syntenic relationship between barley and rice. These gene specific markers provide simple and efficient tools for germplasm characterization and marker-assisted selection for barley malting quality, and ultimately lead to isolation and identification of the major gene(s) controlling multiple quality traits on barley chromosome 5HL.

Details

Title: Dissecting the telomere region of barley chromosome 5HL using rice genomic sequences as references: new markers for tracking a complex region in breeding
Authors/Creators: X-Q Zhang (Author/Creator) - Murdoch University
C. Li (Author/Creator) - Murdoch University
J. Panozzo (Author/Creator) - Department of Primary Industry, DPI Horsham Centre, Horsham, Australia
S. Westcott (Author/Creator) - Agriculture and Food
G. Zhang (Author/Creator) - Zhejiang University
A. Tay (Author/Creator) - Agriculture and Food
R. Appels (Author/Creator) - Murdoch University
M. Jones (Author/Creator)
R. Lance (Author/Creator) - Agriculture and Food
Publication Details: Molecular Breeding, Vol.27, pp.1-9
Publisher: Kluwer Academic Publishers
Identifiers: 991005542515807891
Copyright: © 2010 Springer Science+Business Media B.V.
Murdoch Affiliation: Centre for Comparative Genomics; State Agricultural Biotechnology Centre
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.96 QTL
Web Of Science research areas: Agronomy; Genetics & Heredity; Horticulture; Plant Sciences
ESI research areas: Plant & Animal Science