Journal article
Distribution of nerve fibers and nerve-immune cell association in mouse spleen revealed by immunofluorescent staining
Scientific Reports, Vol.10(1), Art. 9850
2020
Abstract
The central nervous system regulates the immune system through the secretion of hormones from the pituitary gland and other endocrine organs, while the peripheral nervous system (PNS) communicates with the immune system through local nerve-immune cell interactions, including sympathetic/parasympathetic (efferent) and sensory (afferent) innervation to lymphoid tissue/organs. However, the precise mechanisms of this bi-directional crosstalk of the PNS and immune system remain mysterious. To study this kind of bi-directional crosstalk, we performed immunofluorescent staining of neurofilament and confocal microscopy to reveal the distribution of nerve fibers and nerve-immune cell associations inside mouse spleen. Our study demonstrates (i) extensive nerve fibers in all splenic compartments including the splenic nodules, periarteriolar lymphoid sheath, marginal zones, trabeculae, and red pulp; (ii) close associations of nerve fibers with blood vessels (including central arteries, marginal sinuses, penicillar arterioles, and splenic sinuses); (iii) close associations of nerve fibers with various subsets of dendritic cells, macrophages (Mac1+ and F4/80+), and lymphocytes (B cells, T helper cells, and cytotoxic T cells). Our data concerning the extensive splenic innervation and nerve-immune cell communication will enrich our knowledge of the mechanisms through which the PNS affects the cellular- and humoral-mediated immune responses in healthy and infectious/non-infectious states.
Details
- Title
- Distribution of nerve fibers and nerve-immune cell association in mouse spleen revealed by immunofluorescent staining
- Authors/Creators
- D. Hu (Author/Creator) - Hebei Medical UniversityH.A.M. Al-Shalan (Author/Creator) - University of BaghdadZ. Shi (Author/Creator) - Hebei Medical UniversityP. Wang (Author/Creator) - Murdoch UniversityY. Wu (Author/Creator) - West China Hospital of Sichuan UniversityP.K. Nicholls (Author/Creator) - Murdoch UniversityW.K. Greene (Author/Creator) - Murdoch UniversityB. Ma (Author/Creator) - Murdoch University
- Publication Details
- Scientific Reports, Vol.10(1), Art. 9850
- Publisher
- Springer Nature
- Identifiers
- 991005540286607891
- Copyright
- © 2020 Springer Nature Limited
- Murdoch Affiliation
- School of Medical, Molecular and Forensic Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.5 Neuroscience
- 1.5.894 Nicotinic Receptors
- Web Of Science research areas
- Immunology
- ESI research areas
- Immunology