Journal article
Distribution of non-myelinating Schwann cells and their associations with leukocytes in mouse spleen revealed by immunofluorescence staining
European Journal of Histochemistry, Vol.62(2), pp.33-42
2018
Abstract
The nervous system and the immune system communicate extensively with each other in order to maintain homeostasis and to regulate the immune response. The peripheral nervous system (PNS) communicates specifically with the immune system according to local interactions, including the “hardwiring” of sympathetic/parasympathetic (efferent) and sensory nerves (afferent) to lymphoid tissue and organs. To reveal this type of bidirectional neuroimmune interaction at the microscopic level, we used immunofluorescent staining of glial fibrillary acidic protein (GFAP) coupled with confocal microscopy/3D reconstruction to reveal the distribution of non-myelinating Schwann cells (NMSCs) and their interactions with immune cells inside mouse spleen. Our results demonstrate i) the presence of an extensive network of NMSC processes in all splenic compartments including the splenic nodules, periarteriolar lymphoid sheath (PALS), marginal zone, trabecula, and red pulp; ii) the close association of NMSC processes with blood vessels (including central artries and their branches, marginal sinuses, penicillar arterioles and splenic sinuses); iii) the close “synapse-like” interaction/association of NMSC processes with various subsets of dendritic cells (DCs; e.g., CD4+CD11c+ DCs, B220+CD11c+ DCs, and F4/80+ CD11c+ DCs), macrophages (F4/80+), and lymphocytes (B cells, CD4+ T helper cells). Our novel findings concerning the distribution of NMSCs and NMSC-leukocytes interactions inside mouse spleen should improve our understanding of the mechanisms through which the PNS affects cellular- and humoral-mediated immune responses in a variety of health conditions and infectious/non-infectious diseases.
Details
- Title
- Distribution of non-myelinating Schwann cells and their associations with leukocytes in mouse spleen revealed by immunofluorescence staining
- Authors/Creators
- B. Ma (Author/Creator) - Murdoch UniversityC. Yin (Author/Creator) - Hebei Medical UniversityD. Hu (Author/Creator) - Hebei Medical UniversityM. Newman (Author/Creator)P.K. Nicholls (Author/Creator) - Murdoch UniversityZ. Wu (Author/Creator) - Hebei Academy of Agriculture and Forestry SciencesW.K. Greene (Author/Creator) - Murdoch UniversityZ. Shi (Author/Creator) - Hebei Medical University
- Publication Details
- European Journal of Histochemistry, Vol.62(2), pp.33-42
- Publisher
- PAGEPress
- Identifiers
- 991005542318607891
- Copyright
- © 2018 PAGEPress
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
189 File views/ downloads
58 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.6 Immunology
- 1.6.351 Sepsis Immunology
- Web Of Science research areas
- Cell Biology
- ESI research areas
- Biology & Biochemistry