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Open Access CC BY-NC-ND V4.0
Journal article
Diversification of plant specific organellar single-stranded DNA binding genes and their roles in mitochondrial genome rearrangements in Arabidopsis and rice
BMC plant biology, Vol.26(1), 1242
2026
PMID: 42163114
Abstract
Background
Organelle genome stability is essential for plant development and adaptation. Recombination of repetitive sequences in plant mitochondrial genomes is a key factor influencing this stability and is tightly regulated by nuclear-encoded proteins, including plant-specific organellar single-stranded DNA-binding (OSB) proteins. However, the evolutionary history and overall impact of OSB genes on organellar genome stability remain poorly understood.
Results
Here, we identified 182 OSB genes from 59 species across major plant clades. Seed plant OSB genes formed three phylogenetic groups with varying numbers of conserved PDF domains, while chlorophytes, streptophytes, bryophytes, lycophytes, ferns lay outside these groups with lower copies. Using high-fidelity (HiFi) long-read sequencing, we examined the effects of OSB deficiency on organellar genome stability in both eudicots (Arabidopsis) and monocots (rice). In Arabidopsis osb1 and osb4, and rice osb1 mutants, recombination increased at a subset of nearly identical mitochondrial medium-sized repeats but decreased at repeats longer than 1 kb. These repeats displayed asymmetric accumulation of conformations, and a shift in the dominant genome conformation was also observed in some individuals. Moreover, different regions of the mutant mitochondrial genomes showed uneven HiFi read coverage.
Conclusions
Our findings highlight important roles of OSB genes in regulating the stability of plant mitochondrial genomes.
Details
- Title
- Diversification of plant specific organellar single-stranded DNA binding genes and their roles in mitochondrial genome rearrangements in Arabidopsis and rice
- Authors/Creators
- Yingke Hou - Agricultural Genomics Institute at ShenzhenXiaolin Gu - Agricultural Genomics Institute at ShenzhenLiyun Nie - Murdoch UniversityJie Wang - Agricultural Genomics Institute at ShenzhenZhiqiang Wu - Huazhong Agricultural UniversityYi Zou - Agricultural Genomics Institute at Shenzhen
- Publication Details
- BMC plant biology, Vol.26(1), 1242
- Publisher
- Springer Nature
- Grant note
- 2023A1515111029 / Basic and Applied Basic Research Foundation of Guangdong Province (501100021171) 32400191 / National Natural Science Foundation of China (501100001809) 32400191; 32170238 / National Natural Science Foundation of China (http://data.elsevier.com/vocabulary/SciValFunders/501100001809) 110243160001007 / Chinese Academy of Agricultural Sciences (http://data.elsevier.com/vocabulary/SciValFunders/501100005196) RCYX20200714114538196 / Science, Technology and Innovation Commission of Shenzhen Municipality (http://data.elsevier.com/vocabulary/SciValFunders/501100010877) 32170238 / National Natural Science Foundation of China (501100001809) 2023A1515111029 / Basic and Applied Basic Research Foundation of Guangdong Province (http://data.elsevier.com/vocabulary/SciValFunders/501100021171) RCYX20200714114538196 / Science, Technology and Innovation Commission of Shenzhen Municipality (501100010877) 2021QN02N792 / Guangdong Provincial Pearl River Talents Program (http://data.elsevier.com/vocabulary/SciValFunders/100016691) 2021QN02N792 / Guangdong Provincial Pearl River Talents Program (100016691)
- Identifiers
- 991005909777207891
- Copyright
- © The Author(s) 2026.
- Murdoch Affiliation
- School of Medical, Molecular and Forensic Sciences
- Language
- English
- Resource Type
- Journal article
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