Diversity and Bioactivity of Endophytic Actinobacteria Associated with Grapevines

Patanun Kanjanamaneesathian; Anish Shah; Hayley Ridgway; E. Eirian Jones

doi:10.1007/s00284-022-03068-0

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Diversity and Bioactivity of Endophytic Actinobacteria Associated with Grapevines

Journal article

Open access

Peer reviewed

Diversity and Bioactivity of Endophytic Actinobacteria Associated with Grapevines

Patanun Kanjanamaneesathian, Anish Shah, Hayley Ridgway and E. Eirian Jones

Current microbiology, Vol.79(12), pp.390-390

2022

DOI: https://doi.org/10.1007/s00284-022-03068-0

PMID: 36329295

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Abstract

Grapevine trunk diseases (GTDs) are a significant problem for New Zealand viticulture. Endophytic actinobacteria are of interest as potential biocontrol agents due to their ability to inhibit plant pathogens and improve plant growth. However, no studies have investigated the diversity of actinobacteria associated with grapevines in New Zealand vineyards and their bioactivity. Actinobacteria diversity in different ‘Sauvignon blanc’ vine tissues from three vineyards (conventional and organic management, and different vine ages) was assessed using different methods and media. Forty-six endophytic actinobacteria were isolated, with more isolates recovered from roots (n = 45) than leaves (n = 1) and shoot internodes (n = 0). More isolates were recovered from the organic (n = 21) than conventional (n = 8) vineyard, mature (25-year old; n = 21) than young (2-year old; n = 2) vines and using a tissue maceration technique (n = 40). Actinomycete Isolation Agar, International Streptomyces Project 2, and Starch Casein media were effective for actinobacteria isolation. Most of the isolates recovered belonged to Streptomyces, with one isolate identified as Mycolicibacterium. Forty isolates were assessed for antifungal activity and plant growth-promoting (PGP) characteristics. Of these, 13 isolates had antifungal activity against test GTD pathogens (Dactylonectria macrodidyma, Eutypa lata, Ilyonectria liriodendri, Neofusicoccum parvum, and N. luteum). Eighteen isolates exhibited more than one PGP trait; 25siderophore production (n = 25), phosphate solubilization (n = 6), and indole acetic acid production (n = 16). Two strains, Streptomyces sp. LUVPK-22 and Streptomyces sp. LUVPK-30, exhibited the best antifungal and PGP properties. This study revealed the diversity of culturable endophytic actinobacteria from grapevines in New Zealand vineyards and their biocontrol potential against GTD pathogens.

Details

Title: Diversity and Bioactivity of Endophytic Actinobacteria Associated with Grapevines
Authors/Creators: Patanun Kanjanamaneesathian - Lincoln University
Anish Shah - Lincoln University
Hayley Ridgway - Lincoln University
E. Eirian Jones - Faculty of Agriculture and Life Sciences, Lincoln University
Publication Details: Current microbiology, Vol.79(12), pp.390-390
Publisher: Springer US
Grant note: Faculty of Agriculture and Life Sciences, Lincoln University Postgraduate funding Lincoln University
Identifiers: 991005592642007891
Murdoch Affiliation: School of Agricultural Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.97 Plant Pathology; 3.97.556 Microbial Biocontrol
Web Of Science research areas: Microbiology
ESI research areas: Microbiology