Journal article
Diversity detected in commensals at host and farm level reveals implications for national antimicrobial resistance surveillance programmes
Journal of Antimicrobial Chemotherapy, Vol.77(2), pp.400-408
2022
Abstract
Background
A key component to control of antimicrobial resistance (AMR) is the surveillance of food animals. Currently, national programmes test only limited isolates per animal species per year, an approach tacitly assuming that heterogeneity of AMR across animal populations is negligible. If the latter assumption is incorrect then the risk to humans from AMR in the food chain is underestimated.
Objectives
To demonstrate the extent of phenotypic and genetic heterogeneity of Escherichia coli in swine to assess the need for improved protocols for AMR surveillance in food animals.
Methods
Eight E. coli isolates were obtained from each of 10 pigs on each of 10 farms. For these 800 isolates, AMR profiles (MIC estimates for six drugs) and PCR-based fingerprinting analysis were performed and used to select a subset (n = 151) for WGS.
Results
Heterogeneity in the phenotypic AMR traits of E. coli was observed in 89% of pigs, with 58% of pigs harbouring three or more distinct phenotypes. Similarly, 94% of pigs harboured two or more distinct PCR-fingerprinting profiles. Farm-level heterogeneity was detected, with ciprofloxacin resistance detected in only 60% of pigs from a single farm. Furthermore, 58 STs were identified, with the dominant STs being ST10, ST101, ST542 and ST641.
Conclusions
Phenotypic and genotypic heterogeneity of AMR traits in bacteria from animal populations are real phenomena posing a barrier to correct interpretation of data from AMR surveillance. Evolution towards a more in-depth sampling model is needed to account for heterogeneity and increase the reliability of inferences.
Details
- Title
- Diversity detected in commensals at host and farm level reveals implications for national antimicrobial resistance surveillance programmes
- Authors/Creators
- T.J. Laird (Author/Creator)D. Jordan (Author/Creator)Z.Z. Lee (Author/Creator)M. O’Dea (Author/Creator)M. Stegger (Author/Creator)A. Truswell (Author/Creator)S. Sahibzada (Author/Creator)R. Abraham (Author/Creator)S. Abraham (Author/Creator)
- Publication Details
- Journal of Antimicrobial Chemotherapy, Vol.77(2), pp.400-408
- Publisher
- Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy
- Identifiers
- 991005543055907891
- Copyright
- © 2021 The Authors.
- Murdoch Affiliation
- Antimicrobial Resistance and Infectious Disease Laboratory; College of Science, Health, Engineering and Education
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.23 Antibiotics & Antimicrobials
- 1.23.146 Antimicrobial Resistance
- Web Of Science research areas
- Infectious Diseases
- Microbiology
- Pharmacology & Pharmacy
- ESI research areas
- Pharmacology & Toxicology