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Journal article
Diversity of Virulence Factors Associated with West Australian Methicillin-Sensitive Staphylococcus aureus Isolates of Human Origin
BioMed research international, Vol.2016, 8651918
2016
PMID: 27247944
Abstract
An extensive array of virulence factors associated with S. aureus has contributed significantly to its success as a major nosocomial pathogen in hospitals and community causing variety of infections in affected patients. Virulence factors include immune evading capsular polysaccharides, poly-N-acetyl glucosamine, and teichoic acid in addition to damaging toxins including hemolytic toxins, enterotoxins, cytotoxins, exfoliative toxin, and microbial surface components recognizing adhesive matrix molecules (MSCRAMM). In this investigation, 31 West Australian S. aureus isolates of human origin and 6 controls were analyzed for relative distribution of virulence-associated genes using PCR and/or an immunoassay kit and MSCRAMM by PCR-based typing. Genes encoding MSCRAMM, namely, Spa, ClfA, ClfB, SdrE, SdrD, IsdA, and IsdB, were detected in >90% of isolates. Gene encoding α-toxin was detected in >90% of isolates whereas genes encoding β-toxin and SEG were detectable in 50-60% of isolates. Genes encoding toxin proteins, namely, SEA, SEB, SEC, SED, SEE, SEH, SEI, SEJ, TSST, PVL, ETA, and ETB, were detectable in >50% of isolates. Use of RAPD-PCR for determining the virulence factor-based genetic relatedness among the isolates revealed five cluster groups confirming genetic diversity among the MSSA isolates, with the greatest majority of the clinical S. aureus (84%) isolates clustering in group IIIa.
Details
- Title
- Diversity of Virulence Factors Associated with West Australian Methicillin-Sensitive Staphylococcus aureus Isolates of Human Origin
- Authors/Creators
- Charlene Babra Waryah - Curtin UniversityJully Gogoi TiwariKelsi Wells - Curtin UniversityKarina Yui Eto - Curtin UniversityElnaz Masoumi - Curtin UniversityPaul Costantino - Curtin UniversityMichael Kotiw - University of Southern QueenslandTrilochan K. Mukkur - Curtin University
- Publication Details
- BioMed research international, Vol.2016, 8651918
- Publisher
- Hindawi Publishing
- Number of pages
- 10
- Grant note
The authors would like to acknowledge the support of Alain Delhaize, School of Biomedical Sciences, for donation of the S. aureus strains from different hospitals and CHIRI Biosciences Precinct, Curtin University, for the provision of core infrastructure. The first author would also like to thank Curtin University for providing APA (Australian Postgraduate Award) and CRS (Curtin Research Scholarship) in support of the pursuit of her Ph.D.. Final thanks are due to Mrs. Abtar Kaur, an English language editor, for provision of editing services.
- Identifiers
- 991005703766507891
- Copyright
- © 2016 Charlene Babra Waryah et al.
- Murdoch Affiliation
- School of Veterinary Medicine
- Language
- English
- Resource Type
- Journal article
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