Does smooth muscle in an intact airway undergo length adaptation during a sustained change in transmural pressure?

T.K. Ansell; P.K. McFawn; R.A. McLaughlin; D.D. Sampson; P.R. Eastwood; D.R. Hillman; H.W. Mitchell; P.B. Noble

doi:10.1152/japplphysiol.00724.2014

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Does smooth muscle in an intact airway undergo length adaptation during a sustained change in transmural pressure?

Journal article

Peer reviewed

Does smooth muscle in an intact airway undergo length adaptation during a sustained change in transmural pressure?

T.K. Ansell, P.K. McFawn, R.A. McLaughlin, D.D. Sampson, P.R. Eastwood, D.R. Hillman, H.W. Mitchell and P.B. Noble

Journal of Applied Physiology, Vol.118(5), pp.533-543

2015

DOI: https://doi.org/10.1152/japplphysiol.00724.2014

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Abstract

In isolated airway smooth muscle (ASM) strips, an increase or decrease in ASM length away from its current optimum length causes an immediate reduction in force production followed by a gradual time-dependent recovery in force, a phenomenon termed length adaptation. In situ, length adaptation may be initiated by a change in transmural pressure (P-tm), which is a primary physiological determinant of ASM length. The present study sought to determine the effect of sustained changes in Ptm and therefore, ASM perimeter, on airway function. We measured contractile responses in whole porcine bronchial segments in vitro before and after a sustained inflation from a baseline Ptm of 5 cmH(2)O to 25 cmH(2)O, or deflation to -5 cmH(2)O, for similar to 50 min in each case. In one group of airways, lumen narrowing and stiffening in response to electrical field stimulation (EFS) were assessed from volume and pressure signals using a servo-controlled syringe pump with pressure feedback. In a second group of airways, lumen narrowing and the perimeter of the ASM in situ were determined by anatomical optical coherence tomography. In a third group of airways, active tension was determined under isovolumic conditions. Both inflation and deflation reduced the contractile response to EFS. Sustained Ptm change resulted in a further decrease in contractile response, which returned to baseline levels upon return to the baseline Ptm. These findings reaffirm the importance of Ptm in regulating airway narrowing. However, they do not support a role for ASM length adaptation in situ under physiological levels of ASM lengthening and shortening.

Details

Title: Does smooth muscle in an intact airway undergo length adaptation during a sustained change in transmural pressure?
Authors/Creators: T.K. Ansell (Author/Creator) - The University of Western Australia
P.K. McFawn (Author/Creator) - The University of Western Australia
R.A. McLaughlin (Author/Creator) - The University of Western Australia
D.D. Sampson (Author/Creator) - The University of Western Australia
P.R. Eastwood (Author/Creator) - Sir Charles Gairdner Hospital
D.R. Hillman (Author/Creator) - Sir Charles Gairdner Hospital
H.W. Mitchell (Author/Creator) - The University of Western Australia
P.B. Noble (Author/Creator) - The University of Western Australia
Publication Details: Journal of Applied Physiology, Vol.118(5), pp.533-543
Publisher: American Physiological Society
Grant note: Grant ID: 513842
Identifiers: 991005542408207891
Copyright: © 2015 by the American Physiological Society
Murdoch Affiliation: School of Veterinary and Life Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.65 Allergy; 1.65.44 Asthma
Web Of Science research areas: Physiology; Sport Sciences
ESI research areas: Biology & Biochemistry