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Journal article
Durum Wheat cv. Svevo Reference Genome Rel.2.0: A Comprehensive Tool for Wheat Genomics
Plant biotechnology journal, Early View
2026
PMID: 42439890
Abstract
Advancements in plant genome sequencing and assembly have enabled the production of increasingly accurate and contiguous genome sequences. Here, we present the chromosome-level assembly of the durum wheat (Triticum turgidum L. ssp. durum, cv. Svevo) reference genome produced using accurate long-reads, optical mapping and Hi-C. The new assembly (Svevo Rel.2.0) comprises 263 hybrid scaffolds with an N50 value of 112.3 Mb, arranged into 14 contiguous pseudomolecules spanning 10.4 Gb. The Svevo Rel.2.0 genome assembly was annotated using extensive short- and long-read RNA sequencing data obtained from 60 tissue/treatment combinations. The resulting annotation comprises 68 154 high-confidence protein-coding genes, which have been integrated into a comprehensive transcriptome atlas accessible through an eFP browser. Annotation was manually curated for storage protein gene families and for Leucine-Rich Repeat-Containing Receptor genes yielding 3763 LRR-CR loci. The genome assembly's accuracy and completeness were demonstrated by the correct reconstruction of the physical map of Tg1-B (Tenacious glumes 1), a locus controlling the free threshing trait located on chromosome 2B that was not assembled in the previous genome release (Svevo Rel.1.0). A wealth of 6621 QTLs/MTAs from the literature were mapped onto Svevo Rel.2.0 to identify QTL hotspots and trait-specific candidate genes. The ancestry of the durum genome to representative wild emmer populations from North-Eastern and Southern-Levant Fertile Crescent assessed by tracing haplotype transmission patterns revealed a clear mosaic pattern. This new durum reference genome, enhanced with advanced annotation and an expression atlas linked to QTLome data, is the most comprehensive tool available for durum wheat genomics.
Details
- Title
- Durum Wheat cv. Svevo Reference Genome Rel.2.0: A Comprehensive Tool for Wheat Genomics
- Authors/Creators
- Elisabetta Mazzucotelli - Centro di Ricerca per l’OrticolturaCristian Forestan - University of BolognaGina Zastrow-Hayes - Corteva (United States)David Swarbreck - Norwich Research ParkYael Lev-Mirom - University of HaifaEmile Cavalet-Giorsa - King Abdullah University of Science and TechnologyMatteo Bozzoli - University of BolognaAnna Maria Mastrangelo - Cereal Research CentreDaniela Marone - Cereal Research CentreVincent Ranwez - Centre de Coopération Internationale en Recherche Agronomique pour le DéveloppementJohanna Girodolle - Centre de Coopération Internationale en Recherche Agronomique pour le DéveloppementVictor Llaca - Corteva (United States)Kevin Fengler - Corteva (United States)Charlotte Harris - Corteva (United States)Helena Toegelova - Czech Academy of Sciences, Institute of Experimental BotanyPavla Navratilova - Czech Academy of Sciences, Institute of Experimental BotanyPrimetta Faccioli - Centro di Ricerca per l’OrticolturaFrancesca Desiderio - Centro di Ricerca per l’OrticolturaAna Paola Valladares - Centro di Ricerca per l’OrticolturaRachel L. Rusholme-Pilcher - Norwich Research ParkGemy Kaithakottil - Norwich Research ParkManuel Spannagl - Murdoch UniversityHeidrun Gundlach - Helmholtz MunichKlaus F. X. Mayer - Helmholtz MunichVictoria C. Blake - Montana State Univ, Bozeman, MT USAJustin D. Faris - Edward T. Schafer Agricultural Research CenterSteven S. Xu - Western Regional Research CenterTaner Z. Sen - Agricultural Research ServiceEric Yao - Agricultural Research ServiceJulio Isidro y Sanchez - Consejo Superior de Investigaciones CientíficasChunyi Liu - University of BolognaMuhammad A. Farooq - University of BolognaSandra Stefanelli - University of BolognaChiara Cappucci - University of BolognaLeah Oren - University of HaifaTamar Eilam - University of HaifaMario Giorgioni - Forestry Research CentreAlessandra Stella - National Research CouncilBarbara Lazzari - National Research CouncilMassimiliano Lauria - National Research CouncilAldo Ceriotti - National Research CouncilRaul Pirona - National Research CouncilElahe Tavakol - Shiraz UniversityValentyna Klymiuk - University of SaskatchewanJennifer Ens - University of SaskatchewanHarmeet Singh Chawla - University of ManitobaSean Walkowiak - Canada Grain CommissionNicola Pecchioni - Cereal Research CentreFilippo M. Bassi - International Center for Agricultural Research in the Dry AreasMiguel Sanchez-Garcia - ICARDA, Rabat, MoroccoGabriella Sonnante - Institute of Biosciences and BioresourcesPasquale L. Curci - Institute of Biosciences and BioresourcesGiovanni Giuliano - National Agency for New Technologies, Energy and Sustainable Economic DevelopmentGiuseppe Aprea - National Agency for New Technologies, Energy and Sustainable Economic DevelopmentAsher Pasha - University of TorontoAgata Gadaleta - University of Bari Aldo MoroIlaria Marcotuli - University of Bari Aldo MoroStefania Masci - Univ Tuscia, DAFNE, Viterbo, ItalyFrancesco Sestili - Univ Tuscia, DAFNE, Viterbo, ItalySamuela Palombieri - Università degli Studi della TusciaDavide Scaglione - Istituto di Genomica ApplicataMichele Morgante - University of UdineHana Simkova - Czech Academy of Sciences, Institute of Experimental BotanyNicholas J. Provart - Northwest A&F UniversitySimon G. Krattinger - King Abdullah University of Science and TechnologyCurtis J. Pozniak - University of SaskatchewanNathalie Chantret - Centre de Coopération Internationale en Recherche Agronomique pour le DéveloppementAnthony Hall - University of East AngliaAssaf Distelfeld - University of HaifaRoberto Tuberosa - University of BolognaMarco Maccaferri - University of BolognaLuigi Cattivelli - Genomics (United Kingdom)
- Publication Details
- Plant biotechnology journal, Early View
- Publisher
- Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd.
- Number of pages
- 20
- Grant note
- Investimento 1.4-D.D. 1032 17/06/2022; CN00000022 / European Union Next-Generation-EU; European Union (EU) ANR-24-CE20-2538 / Agence Nationale de la Recherche; Agence Nationale de la Recherche (ANR) WheatSecurity DM.142692 / Joint FACCE-JPI Suscrop 2022 031B0190 (de.NBI) / German Federal Ministry of Education and Research; Federal Ministry of Education & Research (BMBF) PanWheatGrain (Grant 20209XY5R9) / Italian Ministry of University and Research PRIN 2020 PRO-WILD-GA-number; 101134965,PRO-GRACE-GA-number; 101094738 / HORIZON EUROPE Framework Programme Discovery-Grant / Natural Sciences and Engineering Research Council of Canada; Natural Sciences and Engineering Research Council of Canada (NSERC); CGIAR Arab Fund for Economic and Social Development: Modernization of Crop Breeding Programs in Arab Countries 4DWheatproject / Genome Canada, Genome Prairie, the Saskatchewan Ministry of Agriculture, Saskatchewan Wheat Development Commission, Alberta Grains, Secan, Agriculture and Agri-Food Canada and the Manitoba Crop Alliance Decoding Biodiversity BBX011089/1 / Biotechnology and Biological Sciences Research Council; UK Research & Innovation (UKRI); Biotechnology and Biological Sciences Research Council (BBSRC)
- Identifiers
- 991005909777707891
- Copyright
- © 2026 The Author(s).
- Murdoch Affiliation
- Centre for Crop and Food Innovation
- Language
- English
- Resource Type
- Journal article
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