Journal article
ELEquant: A developmental framework and validation of forensic and conservation real-time PCR assays
Molecular Biology Reports, Vol.46(2), pp.2093-2100
2019
Abstract
A framework for the development and validation of a qPCR assay for species identification and DNA quantification for conservation and forensic purposes is presented. Elephants are commonly poached for their ivory tusks, which is the primary driving force behind their endangered status. In addition to poaching and trade, habitat loss due to logging and mining has also resulted in loss of elephants. Crimes against animals can be deterred and/or further prosecution sought through testing with forensic genetic techniques. The creation of novel genetic assays can greatly impact wildlife forensic science investigations in identifying the species. Molecular genetic techniques can help enforce conservation efforts; however, they must be properly developed and validated to be of evidentiary quality for court systems. African and Asian elephant buccal cells were used as model in this work. The assay provides a method to differentiate biological fluids of both genera of elephants simultaneously. It can be used for identification of elephant derived products and presents valuable quantification for optimized further testing, such as microsatellite detection.
Details
- Title
- ELEquant: A developmental framework and validation of forensic and conservation real-time PCR assays
- Authors/Creators
- J. Conte (Author/Creator) - Keystone CollegeM.J. Potoczniak (Author/Creator) - Arcadia UniversityC. Mower (Author/Creator) - Armed Forces DNA Identification LaboratoryS.S. Tobe (Author/Creator) - Flinders University
- Publication Details
- Molecular Biology Reports, Vol.46(2), pp.2093-2100
- Publisher
- Springer Netherlands
- Identifiers
- 991005545139707891
- Copyright
- © 2019 Springer Nature B.V.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
Metrics
130 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.64 Phylogenetics & Genomics
- 3.64.71 Genetic Diversity
- Web Of Science research areas
- Biochemistry & Molecular Biology
- ESI research areas
- Molecular Biology & Genetics