Journal article
Eastern grey kangaroo (Macropus giganteus) myofibres. 1. A simplified classification method using two commercially available antibodies
Animal Production Science, Vol.50(6), pp.386-392
2010
Abstract
Skeletal muscles from eastern grey kangaroos (Macropus giganteus) were assessed for myofibre contractile and metabolic characteristics using immunocytochemical and histological staining of serial sections. Myofibre classification using monoclonal antibodies that typically bind to mammalian slow (clone WB-MHC), fast (clone MY-32) and Types 1, 2X and 2B (clone S5 8H2) myosin heavy chains was validated using acid- and alkali-preincubated myofibrillar ATPase, NADH and -glycerophosphate dehydrogenase stains. Myofibres were classified as Type 1 (slow oxidative), Type 2A (fast oxidative-glycolytic), Type 2X/2B (fast glycolytic) or intermediate or transitional myofibre Types 2C (Type 1Type 2A intermediate) and 2AX/B (Type 2AType 2X/2B intermediate). The Type 2 (fast) antibody (clone MY-32) used in the present study did not bind to a subset of fast myofibres in any of the eight kangaroo muscles investigated. These myofibres were identified as Type 2A using clone S5 8H2 and on the basis of the histochemical staining profile. Hence, a simplified immunostaining system using only clones WB-MHC (anti-Type 1) and MY-32 (anti-Type 2X/2B) allowed five myofibre types to be identified based on the staining pattern and intensity of staining for the two antibodies. It is concluded that the myofibres of muscles from kangaroos can be quickly classified into five types using two commercially available antibodies. This method is directly applicable for routine investigations into the myofibre properties of commercially important muscles from the kangaroo musculature and, when combined with enzymatic assays for oxidative and glycolytic activity, will allow for a better understanding of factors influencing the quality of meat from kangaroos.
Details
- Title
- Eastern grey kangaroo (Macropus giganteus) myofibres. 1. A simplified classification method using two commercially available antibodies
- Authors/Creators
- N.B. Spiegel (Author/Creator) - The University of SydneyW.H. Johns (Author/Creator) - University of New EnglandS.D. Sinclair (Author/Creator) - University of New EnglandP.C. Wynn (Author/Creator) - Charles Sturt UniversityJ.M. Thompson (Author/Creator) - University of New EnglandP.L. Greenwood (Author/Creator) - University of New England
- Publication Details
- Animal Production Science, Vol.50(6), pp.386-392
- Publisher
- CSIRO Publishing
- Identifiers
- 991005545119707891
- Copyright
- © 2010 CSIRO
- Murdoch Affiliation
- School of Environmental Science
- Language
- English
- Resource Type
- Journal article
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- 1.255 Musculoskeletal Disorders
- 1.255.1081 Microgravity Muscle Adaptation
- Web Of Science research areas
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- ESI research areas
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